摘要
目的 :在大肠杆菌中表达SARS病毒S2与硫氧还原蛋白 (Trx)的融合蛋白 ,并对其进行血清学鉴定。方法 :克隆编码SARS病毒S2蛋白的基因 ,并在原核表达系统中表达了 6×His S2硫氧还原融合蛋白。用Westernblot分析纯化Trx S2融合蛋白 ,分别与 6份SARS流行前的正常人血清和 6份SARS患者恢复期血清的反应性。结果 :Westernblot分析表明 ,6份SARS患者的恢复期血清均能识别Trx S2融合蛋白 ,且在Mr为 76× 10 3 附近出现特异性的结合带 ;而 6份SARS流行前的正常人血清则不与Trx S2融合蛋白起反应。结论 :本研究获得了纯化的SARS病毒Trx S2融合蛋白 ,它可与SARS患者的恢复期血清产生特异性结合反应 ,为研究SARS病毒感染宿主细胞的过程和制备针对SARS病毒的重组疫苗提供了条件。
AIM: To express S2 protein of SARS virus fused with Trx and then detect its reactivity to the sera from convalescent SARS patients. METHODS: The Trx S2 fusion protein was expressed in E.coli . After purification, the Trx S2 fusion protein was detected by Western blot with 6 serum samples of convalescent SARS patients and 6 serum samples of healthy donors. RESULTS: According to the SDS PAGE analysis , the relative molecular mass ( M r) of the Trx S2 fusion protein is about 76×10 3. The fusion protein could react with all the sera from convalescent SARS patients but not with the sera from healthy donors. CONCLUSION: The Trx S2 fusion protein provides a basis for the research on its role in the course of SARS virus infection of host cells and preparation of recombinant viccine against SARS virus.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2004年第3期257-260,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
教育部SARS攻关基金 (2 0 0 3年度 )
国家重点基础研究发展规划基金(973专项
No.2 0 0 3CB51 4 1 0 0)
国家自然科学基金资助项目(No.30 340 0 1 1 )
