摘要
以百合花丝诱导的胚性愈伤组织为基因转化的受体材料,利用根癌农杆菌介导法将pBIXPTA基因导入百合。组织化学法检测转化后共培养3d的组织块,GUS瞬时表达率可达60%,选择培养20d的组织中约有1%检测到GUS基因的稳定表达。菌液浓度OD600值为0.8左右时侵染效果较好,同时于附加100μmol/L的AS可提高转化效率。PCR分子检测转基因植株部分呈现阳性,初步证明外源基因已整合到百合基因组中。
By using the embryogenic callus from filaments as the gene transformation acceptor, the foreign gene pBIXPTA was transferred into lily via Agrobacterium tumefaciens. The histo-chemical results show that the transient expression of GUS was 60%after 3 d co-culture , and the expression of GUS was 1%after 20 d co-culture. It was better for intrusion with OD600=0.8 Agrobacterium , and the efficiency of intrusion could be increased by supplying 100 μmol/L AS.PCR screening showed that some transgenic plants were positive, which indicated the foreign gene was seemed to integrate into lily's genome.
出处
《上海交通大学学报（农业科学版）》
2004年第2期135-137,167,共4页
Journal of Shanghai Jiaotong University(Agricultural Science)
