期刊文献+

脑缺血再灌注后基质金属蛋白酶-9的早期表达及对血脑屏障的破坏 被引量:17

Early expression of matrix metalloproteinase 9 and blood-brain barrier disruption after transient cerebral ischemia-reperfusion in rats
原文传递
导出
摘要 目的 研究大鼠短暂性脑缺血再灌注对脑组织基质金属蛋白酶 9(MMPs)表达的影响及对血脑屏障的破坏程度 ,探讨两者之间的关系以及在脑缺血损伤中所起的作用。方法 通过制作大鼠脑缺血 /再灌注模型 ,观察脑缺血后不同再灌注时间对脑含水量、血脑屏障通透性及MMPs表达的影响。结果 脑缺血再灌注 6h时 ,大鼠脑组织即有含水量及血脑屏障通透性增加 ,并随着时间推移而呈上升趋势。MMPs主要表达在大鼠缺血侧脑半球内的缺血神经元、血管内皮细胞及嗜中性粒细胞等细胞胞浆中 ,其表达含量于缺血再灌注 6h后开始增加 ,并于再灌注 1~ 2d时达到峰值 ,而该组大鼠缺血对侧脑半球组织内未见MMPs表达 ;假手术组大鼠亦未发现MMPs表达。结论 MMPs的早期表达与血脑屏障通透性的改变密切相关 ,提示MMPs可能参与脑梗死后血管源性水肿的早期形成 ,从而加重脑缺血再灌注损伤。 Objective To study the effects of transient cerebral ischemia-reperfusion (CIR) on the expression of matrix metalloproteinase 9(MM9) in cerebral tissues and the disruption of blood-brain barrier(BBB), and to explore their relationship in addition to their roles in the cerebral ischemic injuries. Methods The effects of different reperfusion time after cerebral ischemia on the water content of brain, the permeability of BBB and the expression of MM9 were investigated after the CIR model was established in rats. Results At 6h after CIR, the cerebral water content and the permeability of BBB were increased with time while MM9 was expressed mainly in the plasma of ischemic neurons and the vascular endothelial cells of the ischemic hemisphere. The expression of MM9 was increased at 6h after reperfusion in the ischemic hemisphere, and reached the peak at 1~2d after reperfusion. However, no expression of MM9 was observed in the hemisphere contralateral to the ischemic side in the CIR group and in the sham-operation group. Conclusion The early expression of MM9 was closely related to changes of the BBB permeability, which indicated that MM9 might be involved in the cerebral edema of vascular origin in the early stage of cerebral infarction, leading to aggravation of the CIR injuries
出处 《中华物理医学与康复杂志》 CAS CSCD 北大核心 2004年第4期203-205,共3页 Chinese Journal of Physical Medicine and Rehabilitation
基金 国家自然科学基金资助项目 (No .39970 935 30 2 71 671 )
关键词 基质金属蛋白酶 缺血性脑损伤 血脑屏障 缺血再灌注 缺血神经元 血管内皮细胞 Matrix metalloproteinase9 Ischemic cerebral injury Blood-brain barrier
  • 相关文献

参考文献7

  • 1Zea Longa E,Weinstein PR,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke,1989,20:84-91.
  • 2Rosenberg GA,Estrada EY,Dencoff JE,et al.Matrix metalloproteinases and TIMPs are associated with blood-brain barrier opening after reperfusion in rat brain.Stroke,1998,29:2189-2195.
  • 3Rosenberg GA,Dencoff JE,Mcguire PG,et al.Injury-induced 92-kilodalton gelatinase and urokinase expression in rat brain.Lab Invest,1994,71:417-422.
  • 4Rosenberg GA,Navratil M,Barone F,et al.Proteolytic cascade enzymes increase in focal cerebral ischemia in rat.J Cereb Blood Flow Metab,1996,16:360-366.
  • 5Heo JH,Lucero J,Abumiya T,et al.Matrix metalloproteinases increase very early during experimental focal cerebral ischemia.J Cereb Blood Flow Metab,1999,19:624-633.
  • 6廖维靖,杨万同,刘淑红,范明,杨运煌,刘买利.线栓阻断大鼠大脑中动脉制作缺血性脑损伤模型的改良[J].中华物理医学与康复杂志,2002,24(6):345-348. 被引量:135
  • 7王社军,蒋传路,康军,胡少山,李永力,葛云龙,杨立庄,戴有利.大鼠脑缺血/再灌注后血脑屏障通透性的改变及转移生长因子β_1的表达[J].中华神经外科杂志,2003,19(1):51-54. 被引量:15

二级参考文献22

  • 1刘晓宏,饶明俐,加藤宏之.实验性脑缺血后CuZn-SOD和Mn-SOD的免疫组化研究[J].中风与神经疾病杂志,1994,11(4):195-198. 被引量:20
  • 2Zea Longa E, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke, 1989, 20: 84-9 1.
  • 3Bederson JB, Pitts LH, Tsuji M, et al. Rat middle cerebral art ery occ-lusion: evaluation of the model and development of a neurological e xamination. Stroke, 1986, 17: 472-476.
  • 4Nagasawa H, Kogure K. Correlation between cerebral blood flow and histologic changes in a new rat model of middle cererbal artery occlusion. S troke, 1989, 20: 1037-1043.
  • 5Laing RJ, Jakubowski J, Laing RW. Middle cerebral artery occlu sion without craniectomy in rats: which method works best? Stroke, 1993, 24: 294 -298.
  • 6Memezawa H, Minamisawa H, Smith ML, et al. Ischemic penumbra in a mod el of reversible middle cerebral artery occlusion in the rat. Exp Brain Res, 199 2, 89: 67-78.
  • 7Aspey BS, Taylor FL, Terruli M, et al. Temporary middle cerebral arte ry occlusion in the rat: consistent protocol for a model of stroke and reperfusi on. Neuropathol Appl Neurobiol,2000, 26: 232-242.
  • 8Nagasawa H;Kogure K.Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion,1989(20).
  • 9Ikede Y;Wang M;Nakazawa S.Simple quantitative evaluation of bloodbrain barrier disruption in vasogenic brain edama,1994(Suppl).
  • 10Rosembery GA;Estrada EY;Encoff JE.Mattix metaiioproteinases and TIMPs are assxiated with BBB opening after reperfusion in rat brain,1998.

共引文献148

同被引文献1746

引证文献17

二级引证文献61

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部