摘要
目的 :探讨 β2 受体激动剂班布特罗 (bambu terol)对哮喘豚鼠支气管肺泡灌洗液 (BALF)淋巴细胞核因子 κB (NF κB)表达的影响。方法 :用卵白蛋白 (ovalbumin ,OA)建立豚鼠哮喘模型 ,BALF分离淋巴细胞 ,采用免疫组化染色方法观察淋巴细胞NF κB表达 ,酶联免疫吸附 (ELISA)法测定淋巴细胞胞浆IκB含量及BALF中白细胞介素 10 (IL 10 )水平。结果 :与模型组相比 ,班布特罗可显著增加BALF中IL 10水平及淋巴细胞胞浆IκB含量 (P <0 .0 5 ) ,降低NF κB胞核阳性细胞百分比 (P <0 .0 5 ) ,而且这一作用可被 β2 受体阻断剂普奈洛尔拮抗 ;但IκB含量及IL 10水平仍低于正常对照组 (P <0 .0 5 ) ,NF κB胞核阳性细胞百分比仍高于正常对照组 (P <0 .0 5 )。Pearson相关分析显示NF κB与IL 10之间呈明显负相关关系 (r =- 0 .5 70 ,P <0 .0 1)。结论 :班布特罗通过抑制IκB降解和增加IL 10水平 ,从而抑制淋巴细胞NF κB表达 ,这可能是其抑制哮喘气道慢性炎症的机制之一。
AIM: To investigate the effects of bambuterol (the agonist of beta2 adrenergic receptor)on expression of nuclear factor kappa B(NF κB) of lymphocytes in bronchoalveolar lavage fluid (BALF) of asthmatic guinea pigs. METHODS: Experimental asthma model of guinea pigs was induced by ovalbumin in vivo, lymphocytes in BALF were separated , the expression of NF κB of lymphocytes was observed by immunohistochemical staining, and inhibitory kappa B(IκB) in lymphocytes and interleukin(IL) 10 in BALF were detected by enzyme linked immunoadsorbent assay (ELISA). RESULTS: Compared with model group, bambuterol could increase IκB in lymphocytes and IL 10 in BALF and decrease the percentage of nuclear NF κB + cells significantly(P< 0.05 ). These effects could be abolished completely by pretreatment with propranolol. While compared with normal control group, IκB and IL 10 of therapeutic group were lower and the percentage of nuclear NF κB + cells was also higher. Furthermore, there was a significantly negative correlation between the percentage of nuclear NF κB + cells and the concentration of IL 10 (r=- 0.570 ,P< 0.01 ). CONCLUSION: Increasing the levels of IκB and IL 10 and inhibiting expression of NF κB subsequently may be one of important mechanisms of bambuterol on airway inflammation of asthma.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2004年第3期285-288,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
北京上地哈佛医药技术有限公司新药研究项目(№ 2 0 0 1BS60 6)
