摘要
以Vero细胞为基质制备马抗狂犬病血清用抗原,以期建立有效、经济、简便的抗原制备方法.用含10%马血清营养液对Vero细胞作适应性培养,接种狂犬病毒,以含1%～3%马血清营养液作维持液培养病毒,于第5,8天收获病毒液,经灭活、浓缩、离心等制成抗原.第5,8天收获病毒滴度可稳定在7.0logLD50/mL以上,灭活抗原具良好的抗原性,用NIH法测定效价达6.0IU/mL以上,可用作抗原生产抗狂犬病血清.
To establish an effective, economical and simple technology for preparing rabies viral antigen that can be used to produce horse anti-rabies serum, rabies virus strain was inoculated in vero cells cultured with medium containing 10% horse serum and was propagated with 199 culture medium containing 1%~3% horse serum. Virus were harvested at the fifth and eighth days and used for preparing antigens by concentration, inactivation and centrifugation. The titers of rabies virus in the culture were reached above 7.0 logLD50/mL .The potency of antigens were higher than 6.0 IU/mL. The antigen is suitable immunising horse for producing rabies immunoglobulin.
出处
《中国病毒学》
CSCD
2004年第3期242-244,共3页
Virologica Sinica
