摘要
通过PCR克隆的方法,获得了分别来自二倍体长穗偃麦草的E基因组和四倍体长穗偃麦草的E_1基因组的4个高分子量麦谷蛋白亚基(HMW-GS)基因启动子的部分序列。序列分析表明,它们之间的同源性较高,两个x型亚基启动子序列之间只有1个碱基的差异,而两个y型亚基启动子序列完全相同,x和y型亚基启动子序列之间的长度和部分碱基位点都有差异。推测四倍体长穗偃麦草中的E_1基因组可能起源于二倍体的E基因组。与来自小麦族的A、B、D和G基因组部分亚基基因的启动子序列比较表明,小麦族的这一区域在进化上是相当保守的,不同基因组来源的序列同源性都在90％以上。经过对这些序列的聚类分析,表明长穗偃麦草的y型HMW-GS基因与其他亚基基因的进化关系较远,而x型亚基基因与一个来自小麦1B染色体的亚基基因关系最近。
The partial promoter regions of HMW glutenin subunit genes were cloned form the genomes E (in diploid
Agropyron elongatum ) and E_1 (in tetraploid Agropyron elongatum ) by PCR approach. There was only one nucleo-
tide acid difference in the promoter sequences of x-type subunits between the two genomes; moreover, the promoter
sequences of the two y-type subunits were completely identical. Although these promoter regions were very similar
to each other, differences still existed in sequence size and the kind of nucleotide acid between the x-type and y-type
subunits. It was speculated that the E_1 genome in tetraploid Agropyron elongatum was probably originated from E
genome in diploid species. The comparisons of these subunits with some of those from A, B, D and G genome of
Triticeae demonstrated that the sequences of their partial promoter regions were conserved and shared a high homol-
ogy more than 90%. The phylogenetic analysis based on the sequences in this region indicated that the y-type HMW
glutenin subunits of Agropyron elongatum species were different from other subunits, whereas the x-type subunits
of them were most closely related to that from the B genome.
出处
《遗传》
CAS
CSCD
北大核心
2004年第3期325-329,共5页
Hereditas（Beijing)
基金
国家973计划(2002CB111300)
863计划(2001AA222091)资助~~
