摘要
以油菜下胚轴和玉米的幼胚为材料,研究19,22,25,28和31℃5个培养温度对含有Bar基因和几丁质酶基因的根癌农杆菌菌株LBA4404的转化效率的影响。结果表明,温度对玉米、油菜的转化效率都有明显的影响,玉米和油菜转化的最佳温度为25℃;25℃条件下,玉米的转化效率与其余4个温度条件下的转化效率有显著的差异;22℃条件下的油菜转化效率和25℃下的差异不显著。
In this article,five co-cultivation temperatures (19,22,25,28 and 31 ℃) were evaluated to determine their effects on rape and maize Agrobacterium tumefaciens transformation efficiency. Rape hypocotyl and maize immature embryo explants were co-cultivated with Agrobacterium tumefaciens LBA4404 containing plas-mids encoding resistance to the herbicide phosphinothricin and chitinase gene. Explants were selected anti-cullus on medium with herbicide phosphinothricin. The results showed that, temperature has a large effects on transformation of rape and maize, Co-culture at 25 ℃ led to the highest number of stable transformed cullus. Although, there have remarkable diversity between 25 ℃ and 19 ℃, 22℃, 28℃ and 31 ℃ on-maize transformation efficiency, on rape transformation efficiency 25℃ and 22℃ have not remarkable diversity.
出处
《华北农学报》
CSCD
北大核心
2004年第2期81-83,共3页
Acta Agriculturae Boreali-Sinica
基金
河南省科技厅科技攻关项目(0124160310)
河南师范大学青年骨干教师基金资助
关键词
温度
根癌农杆菌
转化效率
基因转化
Temperature
Agrobacterium tumefaciens
Transformation efficiency
