摘要
目的 探讨人胎盘组织中是否含有造血干 祖细胞 (HSPC) ,并分析其淋巴细胞亚群的表型特征。方法 将新鲜胎盘制成单个细胞悬液 ,用流式细胞仪分析其有核细胞中HSPC、淋巴细胞及其亚群的表型特征 ,并用流式细胞术 (FCM)或MiniMACS分选胎盘CD34 + 细胞。结果 胎盘CD34 + 细胞百分率是脐带血的 8.8倍 ,CD34+ CD38- 细胞和CD34+ CD38+ 细胞百分率分别为脐带血的 4 .6倍和 11.9倍 ;FCM分选胎盘CD34+ 细胞的回收率和纯度分别为 (6 3.0 5± 10 .14 ) %和 (86 .39± 11.2 7) % ;胎盘中的淋巴细胞总数、T细胞 (CD3+ CD2+ )、B细胞 (CD1 9+ )、Th(CD3+ CD4+ )细胞及Th Ts比值均明显低于脐带血 ,而CD8+ CD2 8- T抑制细胞则明显高于脐带血。结论 人胎盘富含HSPC ,在胎儿期具有重要的造血功能 ,可望成为HSPC移植的重要资源。CD8+ CD2 8- T抑制细胞可能在胎 母免疫耐受中起重要作用。
Objective To study whether human placenta contains hematopoietic stem/progenitor cells (HSPCs),and analyze phenotypes of lymphocyte subpopulations in the placenta. Methods Nucleated cells from fresh human placenta were analyzed for phenotypes of HSPCs and lymphocyte subpopulations by flow cytometry (FCM). And CD_ 34 + cells were sorted from human placenta nucleated cells by FCM or MiniMACS. Results ①CD_ 34 + cells,CD_ 34 +/CD_ 38 + cells,and CD_ 34 +/CD_ 38 - cells from a human placenta were 8.8,4.6 and 11.9 times higher than those from umbilical cord blood (UCB),respectively. ②The yields and purity of CD_ 34 + cells isolated from human placenta by FCM sorting system were (63.05±10.14)% and (86.39±11.27)%,respectively. ③Lymphocytes,T cells (CD_3 +/CD_2 +),B cells(CD_ 19 +),Th cells(CD_3 +,CD_4 +),and Th/Ts ratio in the placenta tissue were apparently lower than those in the UCB,while the CD_8 +/CD_ 28 - T suppressor cells were higher in the placenta than in the UCB. Conclusions Human placenta is rich in HSPCs,and has important hematopoietic function in ontogeny. It is probable that human placenta would be graft resource for HSPCs transplantation. CD_8 +/CD_ 28 - T suppressor cells might play an important role in feto-maternal immunologic tolerance.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2004年第3期175-178,共4页
Chinese Journal of Hematology
基金
贵州省科技发展计划资助项目 (2 0 0 2 2 0 5 1)
