摘要
将纯化IBDV组织毒免疫的BALB/C鼠脾细胞与SP2/0骨髓瘤细胞融合,用ELISA、病毒中和试验检测分泌抗体,获得了六株稳定分泌抗IBDV单克隆抗体的杂交瘤细胞,命名为NIB—1,NIB—2,NIB—3,NIB—4,NIB—5,NIB—6;六株单抗均具ELISA特性和免疫沉淀特性,亚类鉴定表明,前四株属于IgG_(2a),后二株属于IgG_1。杂交瘤细胞冻存六个月后复苏,均能稳定分泌特异性单克隆抗体。
Six stable hybridoma cells line, secreting specific neutralizing monoclonal antibodies to infectious bursal disease virrus,were established by fusions between the murine myeloma cell sp2/o and spleen cells of BALB/C mice immunized with IBDV of chickens,and designated as NIB1 to NIB6 which were screened by Enzyme Linked Immunosorbent Assay(ELISA)and virus neutralization test. Among them,the former four cell lines belong to subclass IgG2a and the latter IgG1.
关键词
纯化
LBDV
杂交瘤
单克隆抗体
purified IBDV
hybridoma technique
virus neutraligation test
identification of subclass.
