摘要
目的 :测定不同工艺提取的龙血竭中对羟基苯甲酸乙酯的含量。方法 :样品用甲醇溶解 ,经TLC分离甲醇洗脱后 ,再以C18键合硅胶为固定相 ,乙腈 1%冰醋酸 ( 31∶6 9)为流动相 ,检测波长 2 5 7nm ,外标法定量。结果 :对羟基苯甲酸乙酯在 0 .2 0 6～ 4 .12ng有良好线性关系 ,r=0 .9998,方法平均回收率为 97.2 % ,RSD为 1.4 %。结论
Objective: Quantitative determination was made of ethyl p hydroxybenzoate in Dracaena cochinchinensis extracted with two technologies. Method:The sample was resolved with methanol and isolated by TLC, purged with methanol. Tge sample solution was chroma to graphed on a C 18 column with acetonitrile 1% acetic acid (31∶69) as mobile phase, detecting at 257 nm and content was calculated with external standard method. Result: The standard curves of ethyl p hydroxybenzoate were linear in the range of 0.206～4.12 ng, r =0.999 8. The average recovery was 97.2% and RSD was 1.4%. Conclusion:The content of ethyl p hydroxybenzoate in D. cochinchinensis extracted with heating tree technongy is higher than that with traditiond technology.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2004年第4期323-325,共3页
China Journal of Chinese Materia Medica
基金
国家科技创新计划 ( 0 2CJ 15 0 1 0 8)
关键词
龙血竭
对羟基苯甲酸乙酯
含量测定
提取工艺
高效液相色谱法
Dracaena cochinchinensis
heating-tree technongy
traditional technology
HPLC
ethyl-p-hydroxybenzoate
