摘要
本文采用人胎盘后血为原料,经Sephadex G 200,DEAE-Sephadex A-50层析,等电聚焦电泳,Sepharose 4B负亲和层析技术,从胎盘后血清中分离、纯化出PZP纯品,经8.0％聚丙烯酰胺凝胶圆盘电泳,免疫电泳和双向免疫交叉试验,证实其为单一的成份。
Pregnancy zone protein (PZP) is a serum protein found in pregnancy and after administration of oral contraceptives. PZP is isolated from retroplacental blood. Purification of this protein was achieved in four steps- 1) Gel filtration on Sephadex G 200; 2) Chromatography on DEAE-Sephadex A 50; 3)Iso-electrophoresis; 4 )Chromatography on Sepharose 4B. It was proved that this PZP is a single component by using the techniques of 8. 0% polyacrylamide electrophoresis, immuno-logical electrophoresis and double immunodiffusion.
出处
《白求恩医科大学学报》
CSCD
1993年第4期362-364,共3页
Journal of Norman Bethune University of Medical Science
关键词
妊娠区带蛋白
分离
纯化
电泳
鉴定
Pregnancy zone protein/TP
immunoelectrophoresis/MT
isoelectric focus-ing/MT
