摘要
以G1黑杨幼叶为外植体建立了组织培养高频再生体系 ,对传统的农杆菌介导法进行了改进 ,用来自于菜豆的几丁质酶基因 (CH5B)进行转化 ,经PCR和PCR Southern杂交鉴定 ,证实CH5B基因已整合入G1黑杨核基因组中。
The high frequency regeneration system of Populus deltoids, G1 was established in this study. The CH5B (chtinase 5B) gene was introduced by modified Agrobacterium tumefaciens mediated method into G1. PCR and PCR-Southern tests showed that CH5B gene has been integrated into Populus deltoids genome.
出处
《生物技术通报》
CAS
CSCD
2004年第3期48-51,共4页
Biotechnology Bulletin
关键词
黑杨
根癌农杆菌
几丁质酶基因
转基因杨树
转化
Populus deltoids Agrobacterium tumefaciens Transformation Chitinase gene Transgenic Populus deltoids
