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激光诱导的基因转染方法研究 被引量：2

Study on the Laser-induced Gene Transfection
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摘要 基因转染是研究基因表达、结构和功能的主要实验手段。本文对现有的转染方法进行了分析,并重点探讨了两种激光诱导的基因转染新方法:激光产生冲击波和激光照射纳米微粒的方法,并提出了改进的实验方案。这两种方法都是通过改变细胞质膜通透性实现外源基因的导入,其最大的优点就是对细胞没有损伤。文章的最后说明了基因转染在基因疗法的应用。 Gene tansfection is the main experimental method for studying of gene expressions, structures and functions. Many methods of gene transfection are presented in this article, and two new methods based on laser are emphasized. The application prospect of gene transfection in gene therapy is discussed.
作者 李政 张镇西
机构地区 西安交通大学生物医学信息工程教育部重点实验室生命科学与技术学院生物医学工程研究所
出处 《激光生物学报》 CAS CSCD 2004年第3期219-223,共5页 Acta Laser Biology Sinica
基金 国家自然科学基金资助项目(60178034)
关键词 基因转染 激光诱导 冲击波 纳米微粒 基因疗法 gene transfection laser induced shock wave nano-particle gene therapy
分类号 Q631 [生物学—生物物理学] Q73 [生物学—分子生物学]
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参考文献18

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同被引文献44

  • 1李俊兰,张寒霜,高鹏,王海波.农杆菌介导棉花基因转化的研究[J].华北农学报,2002,17(z1):80-84. 被引量：5
  • 2王自章,张树珍,李杨瑞.T-DNA转移研究进展[J].生命科学研究,2001,5(z1):120-124. 被引量：6
  • 3宣云,黄群策,秦广雍,霍裕平.离子束介导大豆DNA导入小麦后其蛋白质和酯酶同工酶分析[J].激光生物学报,2004,13(4):289-293. 被引量：5
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引证文献2

二级引证文献7

激光生物学报
2004年 第3期

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