摘要
用重组痘苗病毒作载体,在11k、7.5k启动子控制下表达乙型肝炎病毒(HBV)表面抗原(HBsAg)大蛋白、核心抗原(HBcAg)和e抗原(HBeAg)。S和C基因的同时表达与单独表达其表达水平基本接近。大蛋白不分泌,存在于细胞浆内,SDS-PAGE检测未发现中蛋白和主蛋白。核心抗原亦不分泌,但e抗原可分泌,并且表达量大大高于核心抗原。家兔一次性接种重组病毒后,50％以上能产生有关抗体。
The Hepatitis B virus ( HBV ) DNA sequences coding for the large surface protein and HBcAg/HBeAg were inserted into vaccinia virus dual expression vector pJSB1175, such that the HBV S, including PreS gene is under the control of the promoter 11k , while C, including PreC gene is under the control of the promoter 7.5k. TK-143 celis infected with the derived recombinant plasmids in the presence of infections TK+ vaccinia virus ( Tiantan strain) yielded recombinant vaccinia virus that expressed both large surface protein and HBcAg/HBeAg. The large surface protein was not secreted but two polypeptides between 36-45 kilo daltons were revealed in SDS-PAGE following immunoprecipitation by anti-HBs. HBcAg was not secreted either, it remained in the celis. But HBeAg could be secreted into the medium, and was present in much higher amount than HBcAg. Rabbits vaccinated with the recombinant viruses made antibodies that recognized HBsAg and HBcAg epitopes. The additional immunogenicity provided by expression of large protein and HBcAg/HBeAg may be advantageous for the development of an HBV va-ccine.
出处
《病毒学报》
CAS
CSCD
北大核心
1993年第3期209-217,共9页
Chinese Journal of Virology
关键词
乙型肝炎病毒
痘苗病毒
核心抗原
Hepatitis B virus ( HBV ) , Vaccinia virus, HBV large envelope protein, HBV coreantigen, HBV e antigen
