摘要
用PAL(苯丙氨酸解氨酶 )基因正义和反义转化水稻 ,获得了 70株转基因植株。选择正义转化植株 (1s)和反义转化植株 (4a)进行稻瘟病菌 (Magnaporthegrisea)接种 ,针对病原物侵染 ,1s的PAL活性上升更快 ,幅度更大。观察水稻叶片超微结构发现 ,1s的细胞具有更强的抵抗病原菌入侵的能力 ,其过氧化物酶活性也比对照和
By sense and antisense transformation with PAL(phenylalanine ammonia lyase) gene, 70 transgenic rice plant lines were got. When the sense transformant (line 1s) and the antisense one (line 4a) were inoculated with Magnaporthe grisea, the PAL activity in line 1s increased earlier and much more than that in line 4a. The observation on ultrastructure of rice leaf cells showed that the cells of line 1s had stronger resistance to the pathogen invading and higher activity of peroxidase than those of the untransformed and line 4a.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2004年第4期303-308,共6页
Chinese Journal of Rice Science
基金
浙江省自然科学基金资助项目 ( 3 0 12 87)
