摘要
多色荧光原位杂交 (MFISH)在检测核酸序列、染色体和基因方面有着强大功能。由于染色体结构和数目畸变被认为是遗传毒性研究重要的生物学终点 ,而该方法可准确直观地评价暴露于职业、医疗和环境毒物后的染色体异常 ,因此以其为基础的各类方法在人类遗传学、生殖医学、尤其是遗传毒理学中将会得到广泛的应用。
Multicolor fluorescence in situ hybridization, a new promising technology today, offers unparalleled capabilities for detection of nucleic acid sequences, chromosomes and genes. Owing to structural and numerical chromosomal aberrations were considered as important biological end points in genotoxic studies, chromosomal aberrations after exposure to occupational, medical, and environmental toxicants could be accurately and appropriately evaluated via this assay. Consequently, multicolor FISH-based methods could be widespread applied in human genetics, reproductive medicine, especially genetic toxicology. In this paper, the progress in regard was reviewed.
出处
《卫生研究》
CAS
CSCD
北大核心
2004年第3期354-357,共4页
Journal of Hygiene Research
基金
国家基础研究重大项目前期研究专项基金 (No.2 0 0 1CCA0 4 90 0 )
关键词
多色荧光原位杂交
染色体畸变
非整倍体
遗传毒理学
multicolor fluorescence in situ hybridization(MFISH), chromosomal aberrations, aneuploidy, genetic toxicology
