摘要
目的 :建立HPLC法测定口腔溃疡糊剂中盐酸金霉素含量。方法 :色谱柱为KF C18柱 ( 4 .6mm× 2 5 0mm ,10 μm) ;柱温 :4 0℃ ;流动相为水 N ,N二甲基甲酰胺 -乙腈 ( 6 8∶2 7∶5 ) ,含磷酸氢二钠 0 .0 0 6 3mol·L-1和乙二胺四乙酸二钠 0 .0 1mol·L-1,并以 10mol·L-1氢氧化钠溶液调节pH至 8.5 ;检测波长为 380nm。结果 :盐酸金霉素在 10～ 10 0 μg·ml-1的范围内线性关系良好 ,r =0 .9996 ,平均回收率为 99.4 % ,RSD为 1.4 %。结论 :本方法简便 ,快速 ,准确 。
Objective: To establish a HPLC method for the determination of chlortetracycline hydrochloride in stomatic ulcer paste.Method: KF C 18 column ( 4.6 mm×250 mm, 10 μm) was used; the column temperature was maintained at 40 ℃; mobile phase consisted of water N,N dimethylformamide acetonitrile (68∶27∶5), including 0.0063 mol·L -1 disodium hydrogen phosphate dodecahydrate and 0.01 mol·L -1 dinatrii edetatis, and pH was adjusted to 8.5 with 10 mol·L -1 sodium hydroxide; a detecting wavelength was at 380 nm.Result: The linear range of chlortetracycline hydrochloride was within 10 100 μg·ml -1 , r = 0.9996 . RSD was 1.4% . The average recovery was 99.44% .Conclusion: The method was simple, rapid and accurate, and can be used for the quality control of stomatic ulcer paste.
出处
《中国药师》
CAS
2004年第6期429-430,共2页
China Pharmacist
