摘要
目的 研究白细胞介素 -2 (IL 2 )对人宫颈癌HeLa细胞系增殖的影响 ,并探讨其可能的机制。方法 以培养的HeLa细胞进行以下实验 :以MTT比色法 ,测定IL -2对该细胞增殖的剂量效应 ;用流式细胞技术 ,观察IL -2对该细胞周期各阶段DNA含量的影响 ;用激光共聚焦显微镜技术 ,检测IL -2对该细胞内Ca2 + 水平的影响。结果 IL -2 (10 -10 0 0U/ml)可显著促进HeLa细胞的增殖。用流式细胞技术检测细胞DNA的含量发现 ,IL -2 (10 0U/ml)可提高该细胞由G1期进入S期的比例。激光共聚焦法检测到IL -2 (10 0U/ml)可升高细胞内的Ca2 + 浓度。结论 IL -2参与了对宫颈癌细胞增殖的调节 ,其促进增殖作用与该细胞周期DNA含量的变化和Ca2 + 浓度的改变有关。
Objective To investigate the effect of IL-2 on the proliferation of human cervical carcinoma cell line HeLa and explore its possible mechanismMethods Enhancement effect of IL-2 on proliferation of cultured HeLa cells,the effect of IL-2 on DNA content at various periods of cell cycle and on Ca 2+ concentration in the cells were detected and observed by MTT colorimetry,flow cytometry and laser scanning confocal microscope,respectivelyResults IL-2 of 10 to 1000 U/ml significantly stimulated the proliferation of cultured HeLa cellsIL-2(100U/ml)increased ratio of HeLa cells in G 2 and S phases and increased the Ca 2+release from HeLa cellsConclusion IL-2 significantly enhanced the proliferation of HeLa cervical carcinoma cell lineThe enhancement effect of IL-2 on HeLa cell proliferation is possibly related to the altering of DNA content and increased Ca 2+concentration in the cells
出处
《四川医学》
CAS
2004年第6期617-619,共3页
Sichuan Medical Journal
基金
国家自然科学基金资助项目 No :39870 31 9
关键词
白细胞介素-2
宫颈癌
细胞增殖
interleukin-2,cervical carcinoma,cell proliferation
