摘要
目的:建立测定尿碘的全自动分析仪方法,了解样品人群中尿碘的浓度。方法:在标准WS/T107.1-2016砷铈催化分光光度法的基础上优化条件,用全自动碘分析仪分析测定参数,探讨实验注意事项,并将两种方法测定比较了10份尿碘样品的值。结果:全自动尿碘分析仪的检测范围0~1200 μg/L,检出限为2 μg/L,加标回收率94.0%~108.0%,重复进样RSD%在1.2%~2.9%之间。测得尿碘的数值与国标法无统计学意义,其相对偏差小于10%符合国家碘缺乏病参照实验室质量控制要求。结论:所建立的全自动尿碘分析仪方法精密度、准确度和线性相关性较好,比手工法优越。实现了自动化添加试剂,提高了检测速度,避免操作人员和有毒试剂直接接触,能满足基层大批量样品检测的需求。测定了海门区共1777份尿样,数据反映海门区人群中碘含量水平处于较好、大于碘适宜量的水平。
Objective: To establish a method for determining urinary iodine concentration using a fully auto-mated analyzer and to understand the iodine concentration in the sample population. Methods: Op-timizing the conditions based on the standard WS/T107.1-2016 arsenic cerium catalytic spectro-photometry method, analyzing the measurement parameters using a fully automated iodine ana-lyzer, discussing experimental precautions, and comparing the results of measuring 10 urine iodine samples with two methods. Results: The detection range of the fully automated urine iodine ana-lyzer is 0-1200 μg/L, and the detection limit is 2 μg/L. The recovery rate for spiked samples is 94.0% to 108.0%, and the RSD% for replicate injections is between 1.2% and 2.9%. The measured values of urinary iodine showed no statistical significance compared to the national standard method, with a relative deviation of less than 10%, which meets the requirements of the national reference laboratory quality control for iodine deficiency diseases. Conclusion: The established fully automated urine iodine analysis method has good precision, accuracy, and linearity compared to manual methods. It achieves automated reagent addition, improves detection speed, and avoids direct contact between operators and toxic reagents, meeting the needs of bulk sample testing at the grassroots level. A total of 1777 urine samples from Haimen District were analyzed, and the da-ta reflected that the iodine levels in the population in Haimen District were at a good level and above the adequate iodine level.
出处
《分析化学进展》
2023年第4期532-538,共7页
Advances in Analytical Chemistry
