摘要
为研究菊花组培苗脱毒的有效方法,选取生长不正常的菊花为外植体,经植物组织培养技术获得菊花组培苗;然后采用微茎尖脱毒、病毒唑或盐酸吗啉胍培养茎尖培养、热处理脱毒培养、昼夜变温脱毒4种脱毒技术对组培苗进行处理。结果表明:微茎尖脱毒处理是脱除菊花病毒简单有效的方法之一;经移栽通过外部形态观察法检测,100 mg/L的盐酸吗啉胍处理菊花的脱毒效果最好;热处理有利于菊花组培苗脱毒;指示植物检测菊花脱毒方法简单,效果明显。
In order to study the effective method of detoxification of tissue culture seedlings of chrysanthe-mum, the Dendranthema was selected as the experimental material and tissue culture technology was used to obtain tissue culture seedlings of chrysanthemum. Then, four kinds of detoxification techniques were used to detoxify the microstem-tip, to detoxify the microstem-tip, to detoxify the heat treatment, and to detoxify the diurnal temperature. The results showed that the detoxification of microstem tip was one of the simple and effective methods to remove chrysanthemum virus. After transplanting, the external morphological observation method showed that 100mg/L of moroxydine hydrochloride had the best detoxification effect on chrysanthemum. Heat treatment is conducive to the detoxification of chrysanthemum tissue culture seedlings. The detoxification method is simple and effective.
出处
《植物学研究》
2020年第3期185-195,共11页
Botanical Research
基金
云南省省级重点学科建设“生物学”建设(编号IRTSTYN)、云南省高校特色植物资源研究与开发科技创新团队、云南省高校滇中民族植物学重点实验室培育基地项目和王振吉“彝乡英才”项目的资助。
