摘要
为探究不同溶磷解钾复合菌剂对于苜蓿和黑麦草生物学效应的影响,从青藏公路沿线富含磷钾的植物根际土壤中分离纯化溶磷菌和解钾菌,通过可溶性磷、钾的含量测定复筛选出效果最好的三种菌株,即:阿氏芽孢杆菌Bacillus aryabhattai (P09,解磷菌);肠杆菌Enterobacter roggenkampii (K02,解钾菌);芽孢杆菌Bacillus sp. (K04,解钾菌)。将菌种配制成不同比例的菌剂,分别为1:0:0,0:1:0,0:0:1,1:1:0,1:0:1,0:1:1,1:1:1,1:2:1,1:1:2,2:1:1。将复合菌剂施加到苜蓿和黑麦草的土壤中。通过测定植物的各项生理指标,最后确定最佳的复合菌剂的比例。施加菌肥后,所有复合菌剂对苜蓿和黑麦草均有促生作用,其中1:1:0处理比其他处理的总体促生效果更为明显,苜蓿和黑麦草植株的株高、叶片直径(宽)、组织含水量、叶绿素含量、可溶性糖含量和过氧化物酶活性较CK分别增加了15.33%、9.00%、3.35%、78.19%、40.11%、40.77%和35.55%、18.60%、2.47%、33.53%、50.00%、42.66%。实验表明1:1:0比例的菌剂对植物的促生效果最为明显。因此P09菌株和K02菌株对植物的生长有明显的促进作用,可以帮助植物吸收磷钾元素,适宜的在土壤中施加该比例菌剂,将有助于苜蓿、黑麦草和其他植物的生长,对改善青藏公路沿线生态系统有一定的帮助。
In order to participate in the different pictures of the phosphorus-dissolving and potassium-dissolving compound bacteria and the visual effects of black grass animals, phosphate-dissolving bacteria and gum-dissolving bacteria were analyzed from the rhizosphere line of phosphorus and potassium along the wheat-Qinghai-Tibet highway. The effects of supplementary supplements are the best types, namely: Bacillus argentii (P09, phosphate solubilizing bacteria);Enterobacter roggenkampii (K02, resolving canister bacteria);Bacillus spores (P09, solution Phosphorus bacteria) K04, canning bacteria). The strains were prepared into different proportions of bacterial agents, which were 1:0:0, 0:1:0, 0:0:1, 1:1:0, 1:0:1, 0:1:1, 1:1:1, 1:2:1, 1:1:2 and 2:1:1. The compound inoculum was applied to the soil of alfalfa and ryegrass. By measuring various physiological indicators of plants, the optimal ratio of compound microbial agents is finally determined. After the application of bacterial fertilizer, all the compound bacterial agents have a growth-promoting effect on alfalfa and ryegrass. The overall growth-promoting effect of the 1:1:0 treatment is more obvious than other treatments. The plant height and leaf diameter of alfalfa and ryegrass plants (Wide), tissue water content, chlorophyll content, soluble sugar content, and peroxidase activity increased by 15.33%, 9.00%, 3.35%, 78.19%, 40.11%, 40.77% and 35.55%, 18.60%, 2.47%, 33.53%, 50.00%, 42.66% compared with CK, respectively. Experiments show that the 1:1:0 ratio of bacterial agents has the most obvious growth-promoting effect on plants. Therefore, P09 strain and K02 strain have obvious promoting effect on the growth of plants, which can help plants absorb phosphorus and potassium. Appropriate application of this ratio of inoculants in the soil will help the growth of alfalfa, ryegrass and other plants. Improving the ecological system along the Qinghai-Tibet Highway will help.
出处
《植物学研究》
2022年第2期160-170,共11页
Botanical Research
