摘要
通过PCR产物的检测,“金野”紫穗槐和紫穗槐两个品种都含有耐寒基因,从谱带的数量及表现来看,两个品种存在一定的差异,“金野”紫穗槐在同样低温环境下具有更强的耐寒潜在能力,相对于紫穗槐具有一定的优势。品种与对照之间SSR标记点位的指纹信息差别清晰可见,同一品种以及不同品种之间既有一定的联系又存在点位差异,通过对9个点位的比较分析,可初步判定两品种“不同”。为今后新品种的选育建立快速的室内检测方法提供借鉴。
Through the detection of PCR products, it was found that both Amorpha fruticosa “Jinye” and Amorpha fruticosa L. varieties contain cold tolerance genes. From the number and performance of spectral bands, there are certain differences between the two varieties. Amorpha fruticosa “Jinye” has stronger cold tolerance potential under the same low temperature environment, and has certain advantages compared to Amorpha fruticosa L. The difference in fingerprint information of SSR marker points between varieties and controls is clearly visible, and there are both certain connections and differences between the same variety and different varieties. Through comparative analysis of 9 points, it can be preliminarily determined that the two varieties are “different”. It provides reference for the establishment of rapid indoor testing methods for the breeding of new varieties in the future.
出处
《植物学研究》
2023年第4期250-256,共7页
Botanical Research
