摘要
</span><b><span style="font-family:Verdana;">Introduction:</span></b><span style="font-family:Verdana;"></b> Chemotherapy is used to combat tuberculosis, and other microbial infections. Unfortunately, resistance has been reported to a vast majority of currently use drugs.</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">The objective of this study was to search</span><span style="font-family:""><span style="font-family:Verdana;"> for new therapies from plant products for the sustainable management of tuberculosis and <i></span><i><span style="font-family:Verdana;">S. aureus</span></i><span style="font-family:Verdana;"></i></span><i> </i><span style="font-family:Verdana;">infections. <b></span><b><span style="font-family:Verdana;">Materials and Methods:</span></b><span style="font-family:Verdana;"></b> Crude extracts were obtained by sequential maceration of dry powdered plant material in three solvents;hexane, dichloromethane and methanol. Phytochemical screening to identify active constituents in the crude extracts was done by conventional methods. The antimycobacterial and antimicrobial activity on <i></span><i><span style="font-family:Verdana;">M. tuberculosis</span></i><span style="font-family:Verdana;"></i> control strain H37Ra and a clinical isolate of <i></span><i><span style="font-family:Verdana;">S. aureus</span></i><span style="font-family:Verdana;"></i> respectively, was evaluated at eight different concentrations using the microplatealamar blue assay. Cytotoxicity of the active extracts was evaluated on monkey kidney epithelial cells and assessed using the MTT/formazan assay. <b></span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"></b> Twelve crude extracts were obtained, the hexane extract of <i></span><i><span style="font-family:Verdana;">Sanseviera</span></i></span><i><span style="font-family:""> </span></i><i><span style="font-family:Verdana;">liberica</span></i><span style="font-family:""><span style="font-family:Verdana;"></i> rhizomes (P3rH) showed antimycobacterial activity with a minimum inhibitory concentration (MIC) of 1 mg/mL. The hexane and methanol extracts of <i></span><i><span style="font-family:Verdana;">Emilia coccinea</span></i><span style="font-family:Verdana;"></i>, P2H and P2M respectively, showed antimicrobial activity with MICs of 500 μg/mL and 1000 μg/mL respectively. P2M and P2H had selectivity indices of respectively, 0.1046 and 0.2336. <b></span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"></b></span><b> </b><span style="font-family:Verdana;">This study validates the use of <i></span><i><span style="font-family:Verdana;">S. liberica</span></i><span style="font-family:Verdana;"></i> and <i></span><i><span style="font-family:Verdana;">E. coccinea</span></i><span style="font-family:Verdana;"></i> for the traditional management of tuberculosis and <i></span><i><span style="font-family:Verdana;">S. aureus</span></i><span style="font-family:Verdana;"></i> infections, respectively. Furthermore, it provides a base for the purification of the active extracts and generation of leads in the search of alternative drugs for the management of these microbial infections.
</span><b><span style="font-family:Verdana;">Introduction:</span></b><span style="font-family:Verdana;"></b> Chemotherapy is used to combat tuberculosis, and other microbial infections. Unfortunately, resistance has been reported to a vast majority of currently use drugs.</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">The objective of this study was to search</span><span style="font-family:""><span style="font-family:Verdana;"> for new therapies from plant products for the sustainable management of tuberculosis and <i></span><i><span style="font-family:Verdana;">S. aureus</span></i><span style="font-family:Verdana;"></i></span><i> </i><span style="font-family:Verdana;">infections. <b></span><b><span style="font-family:Verdana;">Materials and Methods:</span></b><span style="font-family:Verdana;"></b> Crude extracts were obtained by sequential maceration of dry powdered plant material in three solvents;hexane, dichloromethane and methanol. Phytochemical screening to identify active constituents in the crude extracts was done by conventional methods. The antimycobacterial and antimicrobial activity on <i></span><i><span style="font-family:Verdana;">M. tuberculosis</span></i><span style="font-family:Verdana;"></i> control strain H37Ra and a clinical isolate of <i></span><i><span style="font-family:Verdana;">S. aureus</span></i><span style="font-family:Verdana;"></i> respectively, was evaluated at eight different concentrations using the microplatealamar blue assay. Cytotoxicity of the active extracts was evaluated on monkey kidney epithelial cells and assessed using the MTT/formazan assay. <b></span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"></b> Twelve crude extracts were obtained, the hexane extract of <i></span><i><span style="font-family:Verdana;">Sanseviera</span></i></span><i><span style="font-family:""> </span></i><i><span style="font-family:Verdana;">liberica</span></i><span style="font-family:""><span style="font-family:Verdana;"></i> rhizomes (P3rH) showed antimycobacterial activity with a minimum inhibitory concentration (MIC) of 1 mg/mL. The hexane and methanol extracts of <i></span><i><span style="font-family:Verdana;">Emilia coccinea</span></i><span style="font-family:Verdana;"></i>, P2H and P2M respectively, showed antimicrobial activity with MICs of 500 μg/mL and 1000 μg/mL respectively. P2M and P2H had selectivity indices of respectively, 0.1046 and 0.2336. <b></span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"></b></span><b> </b><span style="font-family:Verdana;">This study validates the use of <i></span><i><span style="font-family:Verdana;">S. liberica</span></i><span style="font-family:Verdana;"></i> and <i></span><i><span style="font-family:Verdana;">E. coccinea</span></i><span style="font-family:Verdana;"></i> for the traditional management of tuberculosis and <i></span><i><span style="font-family:Verdana;">S. aureus</span></i><span style="font-family:Verdana;"></i> infections, respectively. Furthermore, it provides a base for the purification of the active extracts and generation of leads in the search of alternative drugs for the management of these microbial infections.
作者
Moses Samje
Brice Tchoufack
Paulin Ngoufo
Henry M. Dilonga
Moses Samje;Brice Tchoufack;Paulin Ngoufo;Henry M. Dilonga(Department of Biomedical Sciences, University of Bamenda, Bamenda, Cameroon;Department of Biochemistry, University of Bamenda, Bamenda, Cameroon;Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon)
