摘要
Recent ethnopharmacological data cited <i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> as one of the most widely used medicinal plants in the management of salmonellosis in Benin. However,</span><span> </span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">data related to its activity on non-typhoidal Salmonella spp are limited. This study aimed to assess the antibacterial activity of </span><i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> on multidrug-resistant </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">. Ethanolic and aqueous extracts of </span><i><span style="font-family:Verdana;">S. siamea</span></i><span style="font-family:Verdana;"> were tested for their antibacterial activity on four multidrug-resistant </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">: </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;"> Typhimurium ATCC 14028 and three </span><i><span style="font-family:Verdana;">Salmonella </span></i><span style="font-family:Verdana;">spp</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">. </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">isolated from animals intended for human consumption in Benin. Well diffusion technique combined with the determination by microdilution of Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration (MIC) were used for antibacterial testing. From antibacterial testing, inhibition diameters of the extracts</span><i> </i><span style="font-family:Verdana;">ranged from 7 to 11 mm, for the susceptible strains. Colistin (reference antibiotic) was active on all </span><i><span style="font-family:Verdana;">Salmonella spp.</span></i><span style="font-family:Verdana;"> with inhibition diameters between 18 and 19 mm. The MICs ranged from 3.125 to 25 mg/ml while MBCs of the extracts are greater than 100 mg/ml, so none of the extracts have antibacterial power (p.a). From these results, it appears that </span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">the </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">use of </span><i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> in the traditional treatment of salmonellosis is justified. These results must be valued in the development of anti-</span><i><span style="font-family:Verdana;">salmonella</span></i><span style="font-family:Verdana;"> phytomedicines.</span></span></span></span>
Recent ethnopharmacological data cited <i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> as one of the most widely used medicinal plants in the management of salmonellosis in Benin. However,</span><span> </span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">data related to its activity on non-typhoidal Salmonella spp are limited. This study aimed to assess the antibacterial activity of </span><i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> on multidrug-resistant </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">. Ethanolic and aqueous extracts of </span><i><span style="font-family:Verdana;">S. siamea</span></i><span style="font-family:Verdana;"> were tested for their antibacterial activity on four multidrug-resistant </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">: </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;"> Typhimurium ATCC 14028 and three </span><i><span style="font-family:Verdana;">Salmonella </span></i><span style="font-family:Verdana;">spp</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">. </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">isolated from animals intended for human consumption in Benin. Well diffusion technique combined with the determination by microdilution of Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration (MIC) were used for antibacterial testing. From antibacterial testing, inhibition diameters of the extracts</span><i> </i><span style="font-family:Verdana;">ranged from 7 to 11 mm, for the susceptible strains. Colistin (reference antibiotic) was active on all </span><i><span style="font-family:Verdana;">Salmonella spp.</span></i><span style="font-family:Verdana;"> with inhibition diameters between 18 and 19 mm. The MICs ranged from 3.125 to 25 mg/ml while MBCs of the extracts are greater than 100 mg/ml, so none of the extracts have antibacterial power (p.a). From these results, it appears that </span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">the </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">use of </span><i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> in the traditional treatment of salmonellosis is justified. These results must be valued in the development of anti-</span><i><span style="font-family:Verdana;">salmonella</span></i><span style="font-family:Verdana;"> phytomedicines.</span></span></span></span>
