摘要
<span style="font-family:;" "=""><span style="font-family:Verdana;">The uropathogenic </span><i><span style="font-family:Verdana;">Staphylococcus</span></i></span><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">saprophyticus</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> is reported severally to be resistant to the drugs often used empirically for treatment of urinary tract infections (UTIs). Their ability to exhibit resistance to multiple drugs is a great de</span><span style="font-family:Verdana;">al of threat to successes recorded in the management of UTIs cause</span><span style="font-family:Verdana;">d by this pathogen. Lactic acid bacteria (LAB) have been demonstrated to exhibit an</span><span style="font-family:Verdana;">timicrobial activities but studies about their prospect against multi</span><span style="font-family:Verdana;">-drug resistant </span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">saprophyticus</span></i><span style="font-family:Verdana;"> are quite few. This study therefore investigated acti</span><span><span style="font-family:Verdana;">vities of LAB against the multi-drug resistant </span><i><span style="font-family:Verdana;">S</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">saprophyticus</span></i><span style="font-family:Verdana;"> recover</span></span><span style="font-family:Verdana;">ed fro</span><span style="font-family:Verdana;">m urine samples of symptomatic women. The three differ</span><span style="font-family:Verdana;">ent species of LAB </span><span><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">Lactobacillus</span></i></span></span><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">fermentum</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> BTA 62, </span><i><span style="font-family:Verdana;">Lactobacillus</span></i></span><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">johnsonii</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> BTA 86 and </span><i><span style="font-family:Verdana;">Weis</span></i></span><i><span style="font-family:Verdana;">sella</span></i><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">confusa</span></i><span style="font-family:Verdana;"> BTA 40) previously isolated and identified by 16S rRNA s</span><span style="font-family:;" "=""><span style="font-family:Verdana;">e</span><span style="font-family:Verdana;">quencing were selected based on their history of antimicrobial activities. Their</span><span style="font-family:Verdana;"> m</span><span style="font-family:Verdana;">etabolites were employed in the antagonistic assays against six (6) mu</span><span style="font-family:Verdana;">lti-drug resista</span><span style="font-family:Verdana;">nt test pathogens recovered fro</span><span style="font-family:Verdana;">m urine samples of symptomatic, non-pregnant women attending clinics in Lagos, Nigeria and the control (</span><i><span style="font-family:Verdana;">S</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">saprophyticus</span></i><span style="font-family:Verdana;"> subs bovis strain DSM 18669) following standard procedures. </span><span style="font-family:Verdana;">The pathogens showed resistances to almost all the antibiotics except</span><span style="font-family:Verdana;"> levofloxaci</span><span style="font-family:Verdana;">n, ciprofloxacin, imipenem while the control showed resistance to thr</span><span style="font-family:Verdana;">ee. The LAB, </span><i><span style="font-family:Verdana;">L.</span></i></span><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">fermentum</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> inhibited five (83.3%) of the pathogens with zone diameter of 12 - 17 mm, followed by </span><i><span style="font-family:Verdana;">W</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">confusa</span></i><span style="font-family:Verdana;"> inhibiting three (50%) with 15 - 17 mm. </span><i><span style="font-family:Verdana;">Lactobacillus</span></i></span><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">johnsonii</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> on the other hand, inhibited a pathogen and the control with zones of 13 mm and 14 mm respectively. In conclusion, the extracted metabolites of LAB inhibited the growth of multi-drug resistant clinical isolates of uropathogenic </span><i><span style="font-family:Verdana;">S</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">saprophyticus</span></i><span style="font-family:Verdana;"> and may therefore be potent alternatives to antibiotics.</span></span>
<span style="font-family:;" "=""><span style="font-family:Verdana;">The uropathogenic </span><i><span style="font-family:Verdana;">Staphylococcus</span></i></span><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">saprophyticus</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> is reported severally to be resistant to the drugs often used empirically for treatment of urinary tract infections (UTIs). Their ability to exhibit resistance to multiple drugs is a great de</span><span style="font-family:Verdana;">al of threat to successes recorded in the management of UTIs cause</span><span style="font-family:Verdana;">d by this pathogen. Lactic acid bacteria (LAB) have been demonstrated to exhibit an</span><span style="font-family:Verdana;">timicrobial activities but studies about their prospect against multi</span><span style="font-family:Verdana;">-drug resistant </span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">saprophyticus</span></i><span style="font-family:Verdana;"> are quite few. This study therefore investigated acti</span><span><span style="font-family:Verdana;">vities of LAB against the multi-drug resistant </span><i><span style="font-family:Verdana;">S</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">saprophyticus</span></i><span style="font-family:Verdana;"> recover</span></span><span style="font-family:Verdana;">ed fro</span><span style="font-family:Verdana;">m urine samples of symptomatic women. The three differ</span><span style="font-family:Verdana;">ent species of LAB </span><span><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">Lactobacillus</span></i></span></span><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">fermentum</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> BTA 62, </span><i><span style="font-family:Verdana;">Lactobacillus</span></i></span><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">johnsonii</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> BTA 86 and </span><i><span style="font-family:Verdana;">Weis</span></i></span><i><span style="font-family:Verdana;">sella</span></i><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">confusa</span></i><span style="font-family:Verdana;"> BTA 40) previously isolated and identified by 16S rRNA s</span><span style="font-family:;" "=""><span style="font-family:Verdana;">e</span><span style="font-family:Verdana;">quencing were selected based on their history of antimicrobial activities. Their</span><span style="font-family:Verdana;"> m</span><span style="font-family:Verdana;">etabolites were employed in the antagonistic assays against six (6) mu</span><span style="font-family:Verdana;">lti-drug resista</span><span style="font-family:Verdana;">nt test pathogens recovered fro</span><span style="font-family:Verdana;">m urine samples of symptomatic, non-pregnant women attending clinics in Lagos, Nigeria and the control (</span><i><span style="font-family:Verdana;">S</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">saprophyticus</span></i><span style="font-family:Verdana;"> subs bovis strain DSM 18669) following standard procedures. </span><span style="font-family:Verdana;">The pathogens showed resistances to almost all the antibiotics except</span><span style="font-family:Verdana;"> levofloxaci</span><span style="font-family:Verdana;">n, ciprofloxacin, imipenem while the control showed resistance to thr</span><span style="font-family:Verdana;">ee. The LAB, </span><i><span style="font-family:Verdana;">L.</span></i></span><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">fermentum</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> inhibited five (83.3%) of the pathogens with zone diameter of 12 - 17 mm, followed by </span><i><span style="font-family:Verdana;">W</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">confusa</span></i><span style="font-family:Verdana;"> inhibiting three (50%) with 15 - 17 mm. </span><i><span style="font-family:Verdana;">Lactobacillus</span></i></span><i><span style="font-family:;" "=""> </span></i><i><span style="font-family:Verdana;">johnsonii</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> on the other hand, inhibited a pathogen and the control with zones of 13 mm and 14 mm respectively. In conclusion, the extracted metabolites of LAB inhibited the growth of multi-drug resistant clinical isolates of uropathogenic </span><i><span style="font-family:Verdana;">S</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">saprophyticus</span></i><span style="font-family:Verdana;"> and may therefore be potent alternatives to antibiotics.</span></span>
作者
Tajudeen Akanji Bamidele
Felix Oluwasegun Alao
Victorious Chisom Ekeh
Muinah Adenike Fowora
Bolanle Alake Adeniyi
Tajudeen Akanji Bamidele;Felix Oluwasegun Alao;Victorious Chisom Ekeh;Muinah Adenike Fowora;Bolanle Alake Adeniyi(Molecular Biology & Biotechnology Department, Nigerian Institute of Medical Research, Yaba-Lagos, Nigeria;Department of Biological Sciences, Bells University of Technology, Ota, Ogun State, Nigeria;Department of Microbiology, Faculty of Science, University of Lagos, Akoka, Lagos, Nigeria;Pharmaceutical Microbiology Department, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria)
