摘要
目的 比较肾肿瘤患者血清中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、肾损伤分子-1(KIM-1)及胱抑素(CysC)表达水平及临床意义.方法 选择浙江大学国际医院2016年1月至2017年3月确诊的120例肾肿瘤患者为研究组,并选择同期60例体检志愿者为对照组.采用ELISA法测定血清NGAL、KIM-1水平,电化学发光法测定血清CysC水平,比较各组NGAL、KIM-1、CysC水平差异,分析其相关性.结果 研究组患者血清NGAL、KIM-1、CysC水平显著高于对照组;恶性肿瘤患者血清NGAL、KIM-1、CysC水平显著高于良性肿瘤患者;1级肿瘤血清NGAL、KIM-1、CysC水平低于2级、3级和4级肿瘤患者,Furhman分级越高血清NGAL、KIM-1、CysC水平越高,差异均有统计学意义(P<0.05).肾损伤程度与血清NGAL、KIM-1、CysC水平存在密切相关性,血清NGAL表达与KIM-1、CysC水平呈正相关(r=0.812、0.765,P<0.05),KIM-1表达与CysC水平呈正相关(r=0.832,P<0.05).多元回归分析显示,血清NGAL、KIM-1、CysC表达水平与肾肿瘤患者Furhman分级密切相关(P<0.05).结论 肾肿瘤患者血清NGAL、KIM-1、CysC水平显著升高,临床分期越高,其表达水平越高,具有密切的相关性,可作为肾肿瘤的诊断标志物.
Objective To investigate the expression and clinical significance of serum neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1) and serum cystain C (CysC) in patients with renal tumor. Methods A total of 120 patients with renal tumor diagnosed in International Hospital of Zhejiang University from January 2016 and March 2016 was selected as the research object, and 60 patient medical volunteers were selected as control. The levels of NGAL and KIM-1 were determined by enzyme-linked immunosorbent assay ( ELISA) method, and the levels of serum CysC were determined by electrochemical luminescence method. The levels of NGAL, KIM-1, and CysC were compared, and their relevance was analyzed. Results The levels of NGAL, KIM-1, and CysC in research object were signifi-cantly higher than the control group; the levels of NGAL, KIM-1, and CysC in malignant tumor patients were significantly higher than the benign tumor;the levels of NGAL, KIM-1, and CysC in one level of ser-um tumor were lower than the two levels, three levels, and four levels of tumor patients. With the more Furhman grading, the levels of NGAL, KIM-1, and CysC were higher, the differences were statistically sig-nificant (P<0. 05). The levels of serum NGAL, KIM-1, and CysC were significantly related to renal dam-age degree. The levels of serum NGAL were significantly positively related to KIM-1 and CysC ( r= 0. 812 and 0. 765 ) . The levels of serum KIM-1 expression were significantly positively related to CysC ( r =0. 832) (P<0. 05). Multiple regression analysis showed that the levels of serum NGAL, KIM-1, and CysC were significantly related to patients with renal tumor Furhman classification ( P<0. 05 ) . Conclusions The levels of NGAL, KIM-1 and CysC were significantly increased in patients with renal tumor renal tumor, the higher the clinical stage, the higher expressive level, and were significantly related to renal damage de-gree. NGAL, KIM-1, and CysC can be used as the diagnostic markers of renal tumor.
出处
《中国医师杂志》
CAS
2017年第12期1836-1839,共4页
Journal of Chinese Physician
