BACKGROUND Tumor budding(TB)has emerged as a promising independent prognostic biomarker in colorectal cancer(CRC).The prognostic role of TB has been extensively studied and currently affects clinical decision making i...BACKGROUND Tumor budding(TB)has emerged as a promising independent prognostic biomarker in colorectal cancer(CRC).The prognostic role of TB has been extensively studied and currently affects clinical decision making in patients with stage I and II CRC.However,existing prognostic studies on TB in stage III CRC have been confined to small retrospective cohort studies.Consequently,this study investigated the correlation among TB categories,clinicopathological features,and prognosis in stage III-IV CRC to further enhance the precision and individualization of treatment through refined prognostic risk stratification.AIM To analyze the relationship between TB categories and clinicopathological characteristics and assess their prognostic value in stage III-IV CRC to further refine the prognostic risk stratification of stage III-IV CRC.METHODS The clinical data of 547 CRC patients were collected for this retrospective study.Infiltration at the front edge of the tumor buds was counted according to the 2016 International Tumor Budding Consensus Conference guidelines.RESULTS Multivariate Cox proportional hazards regression analysis demonstrated that chemotherapy(P=0.004),clinical stage IV(P<0.001),≥4 regional lymph node metastases(P=0.004),left-sided colonic cancer(P=0.040),and Bd 2-3(P=0.002)were independent prognostic factors in patients with stage III-IV CRC.Moreover,the density of tumor infiltrating lymphocytes was higher in Bd 1 than in Bd 2-3,both in the tumor stroma and its invasive margin.CONCLUSION TB has an independent predictive prognostic value in patients with stage III-IV CRC.It is recommended to complete the TB report of stage III-IV CRC cases in the standardized pathological report to further refine risk stratification.展开更多
Target strength(TS)and circular synthetic aperture sonar(CSAS)images provide essential information for active acoustic detection and recognition of non-cooperative unmanned undersea vehicles(UUVs),which pose a signifi...Target strength(TS)and circular synthetic aperture sonar(CSAS)images provide essential information for active acoustic detection and recognition of non-cooperative unmanned undersea vehicles(UUVs),which pose a significant threat to underwater preset facilities.To access them,we propose an iterative physical acoustics(IPA)-based method to simulate the multiple acoustic scattered fields on rigid surfaces in high-frequency cases.It uses the Helmholtz integral equation with an appropriate Green's function in terms of the Neumann series,and then incorporates the ideas of triangulation and iteration into a numerical implementation.Then two approximate analytic formulae with precise physical meanings are derived to predict the TS and CSAS images of concave targets,respectively.There are no restrictions on the surface's curvature and the order of multiple scattering.The method is validated against the finite element method(FEM)for acoustic scattering from a sphere segment and against an experiment involving an X-rudder UUV's stern.On this basis,we simulate and analyze the TS and CSAS images of an X-rudder UUV.In addition,the influence of the angle of adjacent rudders on the multiple scattering characteristics is discussed.Results show that this method can potentially predict accurate UUV features,especially the multiple scattered features.展开更多
Optimal use of water and fertilizers can enhance winter wheat yield and increase the efficiencies of water and fertilizer usage in dryland agricultural systems.In order to optimize water and nitrogen(N)management for ...Optimal use of water and fertilizers can enhance winter wheat yield and increase the efficiencies of water and fertilizer usage in dryland agricultural systems.In order to optimize water and nitrogen(N)management for winter wheat,we conducted field experiments from 2006 to 2008 at the Changwu Agro-ecological Experimental Station of the Chinese Academy of Sciences on the Loess Plateau,China.Regression models of wheat yield and evapotranspiration(ET)were established in this study to evaluate the water and fertilizer coupling effects and to determine the optimal coupling domain.The results showed that there was a positive effect of water and N fertilizer on crop yield,and optimal irrigation and N inputs can significantly increase the yield of winter wheat.In the drought year(2006–2007),the maximum yield(Ymax)of winter wheat was 9.211 t/hm2for the treatment with 324 mm irrigation and 310 kg/hm2N input,and the highest water use efficiency(WUE)of 16.335 kg/(hm2 mm)was achieved with198 mm irrigation and 274 kg/hm2N input.While in the normal year(2007–2008),the maximum winter wheat yield of 10.715 t/hm2was achieved by applying 318 mm irrigation and 291 kg/hm2N,and the highest WUE was 18.69kg/(hm2 mm)with 107 mm irrigation and 256 kg/hm2N input.Crop yield and ET response to irrigation and N inputs followed a quadratic and a line function,respectively.The optimal coupling domain was determined using the elasticity index(EI)and its expression in the water-N dimensions,and was represented by an ellipse,such that the global maximum WUE(WUEmax)and Ymax values corresponded to the left and right end points of the long axis,respectively.Considering the aim to get the greatest profit in practice,the optimal coupling domain was represented by the lower half of the ellipse,with the Ymax and WUEmax on the two end points of the long axis.Overall,we found that the total amount of irrigation for winter wheat should not exceed 324 mm.In addition,our optimal coupling domain visually reflects the optimal range of water and N inputs for the maximum winter wheat yield on the Loess Plateau,and it may also provide a useful reference for identifying appropriate water and N inputs in agricultural applications.展开更多
In large continental orogens, an important research topic is the behavior of deep crustal and upper mantle deformation, and the flow styles of ductile material. The morphology of the eastern margin of the Tibetan plat...In large continental orogens, an important research topic is the behavior of deep crustal and upper mantle deformation, and the flow styles of ductile material. The morphology of the eastern margin of the Tibetan plateau, adjacent to the Sichuan basin, is characterized by very steep relief with high mountain ranges. The crust beneath this region slows the velocities in the middle and lower crust. We have adopted a relatively dense network to inverse the detailed structure of the crust and upper mantle along the eastern margin of the Tibetan plateau and Sichuan basin, using teleseismic data via receiver function analysis. The results are in-line with the hypothesis that viscous crustal material is flowing beneath the eastern margin of the Tibetan plateau and that this process drives overlying crustal material around the strong and rigid Sichuan basin. When the viscous material hits this obstruction, flows are divided into two or more branches with different directions. The upper part of the upwelling viscous flow produces the pressure to intrude the upper crust, thereby driving uplift of mountain ranges and high peaks. In contrast, the lower part of the downwelling viscous flow produces the pressure to intrude the lower crust and upper mantle to deepen the Moho discontinuity, causing observed crustal thickening.展开更多
BACKGROUND: Heart rate variability refers to the beat-to-beat alteration in heart rate. It is usually a slight periodic variation of R-R intervals. Much information of autonomic nerve system balance can be obtained by...BACKGROUND: Heart rate variability refers to the beat-to-beat alteration in heart rate. It is usually a slight periodic variation of R-R intervals. Much information of autonomic nerve system balance can be obtained by measuring the heart rate variability of patients. It remains to be shown whether heart rate variability can be used as an index for determining the severity of insomnia and cerebral infarction. OBJECTIVE: This study aimed to analyze the correlation for each frequency spectrum parameter of heart rate variability with an insomnia index, as well as the degree of neurological defects in patients with simple cerebral infarction and cerebral infarction complicated by insomnia. The goal was to verify the feasibility of frequency spectrum parameters for heart rate variability as a marker for insomnia and cerebral infarction. DESIGN: A case-control observation. SETTING: Department of Neurology, First Hospital Affiliated to China Medical University. PARTICIPANTS: Sixty inpatients, and/or outpatients, with cerebral infarction were admitted to the 202 Hospital of Chinese PLA between December 2005 and October 2006, confirmed by CT, and recruited to the study. According to the insomnia condition (insomnia is defined by a Pittsburgh Sleep Quality Index score > 7), the patients were assigned to a simple cerebral infarction group and a cerebral infarction complicated by insomnia group, with 30 subjects in each group. Thirty additional subjects, who concurrently received ex- aminations and were confirmed to not suffer from cerebral infarction and insomnia, were recruited into the control group. Written informed consent was obtained from each subject for laboratory specimens. The pro- tocol was approved by the Hospital's Ethics Committee. METHODS: Following admission, each subject's neurological impairment was assessed with the National Institutes of Health Stroke Scale and Pittsburgh Sleep Quality Index. Heart rate variability of each subject was measured with an autonomic nerve analyzer (Weijin Science and Technology Co., Ltd., Taiwan). Each frequency spectrum parameter of heart rate variability was obtained, including very low frequency, low fre- quency, high frequency, total power, R-R interval, and its mean square. In addition, percentage of low fre- quency, high frequency, and ratio of low frequency to high frequency were calculated. For each heart rate frequency spectrum parameter, the difference between groups was analyzed. Moreover, correlations of each frequency spectrum parameter with insomnia and disease condition were analyzed. Data from each index, which were not normally distributed, were processed by logarithmic transformation. The t-test was used for the comparison of intergroup differences. Single-factor linear regression analysis and t-test were used for the analysis of factor-factor correlation and coefficient of correlation, respectively. MAIN OUTCOME MEASURES: ① Differences of scores in the National Institutes of Health Stroke Scale and Pittsburgh Sleep Quality Index between the simple cerebral infarction group and the cerebral in- farction complicated by insomnia group. ② Differences of heart rate variability parameters between the simple cerebral infarction group and the control group. ③ Correlation of heart rate variability parameters, the Pittsburgh Sleep Quality Index score, and the neurological impairment score. RESULTS: Sixty patients and thirty healthy controls were included in the final analysis. ① The scores of the Pittsburgh Sleep Quality Index and the neurological impairment were significantly higher in the cerebral infarction complicated by insomnia group compared to the simple cerebral infarction group (P < 0.05-0.01). ② R-R interval was significantly longer in the simple cerebral infarction group than in the control group, while R-R interval variance and high-frequency band were significantly lower in the simple cerebral infarc- tion group compared to the control group (P < 0.05). ③ For cerebral infarction patients with insomnia, the Pittsburgh Sleep Quality Index score was significantly positively correlated with neurological impairment (r = 0.54, P < 0.01). The low-frequency band, very low-frequency band, high-frequency band, R-R interval variance, total power, R-R interval, and the percentage of high-frequency were significantly negativelycorrelated with the National Institutes of Health Stroke Scale score (r =-0.45 to -0.90, P < 0.05-0.01) and with the Pittsburgh Sleep Quality Index scores (r = -0.56 to -0.36, P < 0.05-0.01). CONCLUSION: Each heart rate variability parameter can be used as an index for assessing dyssomnia and neurologic impairment (r =-0.56 to -0.36, P < 0.05-0.01).展开更多
The incidence of colorectal cancer(CRC)is increasing in China,with high mortality.Here,we aimed to evaluate the latest clinicopathological features and prognostic value of the KRAS/NRAS/BRAF mutation status in CRC pat...The incidence of colorectal cancer(CRC)is increasing in China,with high mortality.Here,we aimed to evaluate the latest clinicopathological features and prognostic value of the KRAS/NRAS/BRAF mutation status in CRC patients in Central China.The clinical data of 1549 CRC patients with stage I-IV disease diagnosed at Union Hospital,Tongji Medical College of Huazhong University of Science and Technology from 2015 to 2017 were collected and analyzed retrospectively.KRAS/NRAS/BRAF mutations were detected by real-time quantitative polymerase chain reaction(q-PCR)in 410 CRC patients,with mutation frequencies of KRAS,NRAS and BRAF of 47.56%,2.93%and 4.15%,respectively.The gene mutation status and clinicopathological characteristics of 410 patients with CRC who underwent qPCR were analyzed.The KRAS and BRAF gene mutations were related to the pathological differentiation and number of metastatic lymph nodes.The BRAF gene mutation was also associated with cancer thrombosis in blood vessels.Cox regression analysis showed that there was no statistically significant difference in the overall survival(OS)between patients with KRAS,NRAS mutants and wild-type CRC patients,while the BRAF gene mutation was negatively correlated with the OS rate of CRC patients.It is suggested that the BRAF gene mutation may be an independent risk factor for the prognosis of CRC.展开更多
A numerical triangulation and transformation into the time domain of a Kirchhoff approximation(KA)method is proposed for the modeling of bistatic scattering from an underwater non-penetrable target.The time domain sol...A numerical triangulation and transformation into the time domain of a Kirchhoff approximation(KA)method is proposed for the modeling of bistatic scattering from an underwater non-penetrable target.The time domain solution in this approximation can be split up into two parts:the solution of reflected field,contributing around the specular direction,and the solution of shadow radiation,contributing around the forward direction.An average solution in the time domain satisfying the reciprocity principle is presented.The solution is expressed in terms of non-singular functions.The proposed method is validated against a normal mode method for bistatic scattering from a rigid sphere.Moreover,the reflected and shadow highlights on the surface of the sphere are shown to verify the integration surface of the reflected field and shadow radiation.It is also tested against a finite element method and an experiment involving a scaled Benchmark Target Strength Simulation Submarine model.The time-angle bistatic spectra for the model are evaluated by the direct and transformed average solutions of KA,and the former accelerates its speed of calculation.The results are good,and show that this method can be used to predict the bistatic scattered field of a non-penetrable target.展开更多
A new acrylic ester polymer YWB-7 resin was prepared and characterized. The properties of YWB-7 resin were compared with those of the commercial Amberlite XAD-7, Diaion HP2MG and hypercrosslinked macroporous polymer N...A new acrylic ester polymer YWB-7 resin was prepared and characterized. The properties of YWB-7 resin were compared with those of the commercial Amberlite XAD-7, Diaion HP2MG and hypercrosslinked macroporous polymer NDA-150 resins. Both surface area and micropore area of YWB-7 resin were bigger than those of XAD-7 resin and HP2MG resin. The YWB-7 resin was successfully employed to recycle 5-sodiosulfoisophthalic acids (SIPA) from its solutions with and without methanol.展开更多
Dissipation kinetics of chlorpyrifos in the near-neutral paddy soils of a vegetable cropping system under greenhouse, screenhouse and field conditions was studied using a rapid analytical method. The recoveries of chl...Dissipation kinetics of chlorpyrifos in the near-neutral paddy soils of a vegetable cropping system under greenhouse, screenhouse and field conditions was studied using a rapid analytical method. The recoveries of chlorpyrifos were between 86.5% and 105.5% with relative standard deviations for repeatability between 6.6% and 9.1% at fortification levels of 0.01, 0.1 and 1 mg/kg in the soil. The limit of detection of the method was 0.004 mg/kg and the limit of quantification was found to be 0.01 mg/kg. The dissipation rates of chlorpyrifos followed the first-order kinetics and the half-life was 6.96, 6.04 and 5.20 days in the soil under greenhouse, screenhouse and field conditions, respectively. The dissipation rates of chlorpyrifos in the soil varied with different cultivation conditions. Chlorpyrifos in the soil dissipated slower in a greenhouse and screenhouse than in the open field, which was likely attributed to the hermetic environment in the greenhouse and screenhouse.展开更多
Organic carbon(OC)source attribution for cascade reservoir sediments has been identified as a critical gap in understanding the effective carbon sink of inland waters.In this study,nine sediment cores were collected f...Organic carbon(OC)source attribution for cascade reservoir sediments has been identified as a critical gap in understanding the effective carbon sink of inland waters.In this study,nine sediment cores were collected from cascade reservoirs in the Wujiang and Pearl Rivers.We analyzed lignin phenol(Λ8),total organic carbon(TOC)content,and stable carbon isotopic composition(δC)of the sediments,focusing on the changes of terrigenous OC,and the variation of OC source in cascade reservoir sediments after damming.Our results showed that theΣ8 and TOC contents decreased from upstream to downstream reservoirs,indicating the significant interception of terrigenous OC by cascade damming.Additionally,theΛ8 content in the Pearl River reservoir sediments was much higher than that in the Wujiang River.From the three-end-member mixing model,we estimated that OC in reservoir sediments mainly comes from soil and plankton.After damming,the proportion of plankton OC in TOC slightly increased in seasonal and annual regulation reservoirs due to the limnetic evolution of the reservoir.These findings suggest that the cascade damming increases the interception capacity of the river to terrigenous OC and nutrients,and that slowing of water velocity caused by damming affected primary productivity and fluvial carbon cycling.展开更多
Objective: To investigate human cytomegalovirus infec-tion and genetic variations in glycoprotein B(gB) inliver transplant recipients in south-east China.Methods:EDTA-blood samples were obtained from 21liver transplan...Objective: To investigate human cytomegalovirus infec-tion and genetic variations in glycoprotein B(gB) inliver transplant recipients in south-east China.Methods:EDTA-blood samples were obtained from 21liver transplant recipients. The semi-nested PCR wasused to amplify a region of high sequence variabilityin the gB gene of human cytomegalovirus (HCMV)followed by direct sequence analysis.Results: Out of the 21 liver transplant recipients, 5were proved HCMV positive 62 to 180 days aftertransplantation. The nucleotide and encoded aminoacid sequences were compared with published se-quences of AD169 and Towne laboratory strains.Within the region sequenced, 2 out of 5 strains pos-sessed a peptide configuration similar to that of strainAD169, while another 2 strains displayed a peptideconfiguration similar to that of strain Towne. Onestrain had amino acid substitution, which was differ-ent from those of both AD169 and Towne in thecleavage site.Conclusion: Our results provide molecular epidemio-logical data for HCMV strains circulating among trans-plant recipients in south-east China.展开更多
Objective: To explore cytomegalovirus (CMV) infec-tion in recipients of liver transplantation (LT).Methods: 30 recipients of LT were screened for theappearance of CMV infection by using ELISA to testanti-CMV-Ab from s...Objective: To explore cytomegalovirus (CMV) infec-tion in recipients of liver transplantation (LT).Methods: 30 recipients of LT were screened for theappearance of CMV infection by using ELISA to testanti-CMV-Ab from serum samples and using im-munohistochemistry method to test CMV antigen ex-pression and nested-PCR to amplify CMV-DNA fromblood samples.Results: Four of 243 samples taken from 30 recipientscame out positive of anti-CMV IgC; and anti-CMVIgM with a positive rate of 100% and 1.6% respec-tively. 85 samples resulted in CMV antigen expression(35.0%) with the average antigen index being 4.2±3.1/5×10~4 WBC. Besides, 99 samples were found tobe positive by nested-PCR with a positive rate of40.7%. 61 samples were found to be simultaneously pos-itive in test of CMV antigen and DNA, with a rate of25.1%.Conclusions: Infection of CMV is common in recipi-ents of LT. Simultaneous screening of anti-CMV-Ab, CMV-Ag and CMV-DNA after liver transplanta-tion is very important for early diagnosis of CMV in-fection.展开更多
Emerging evidence suggested that zinc finger protein 831(ZNF831)was associated with immune activity and stem cell regulation in breast cancer.Whereas,the roles and molec-ular mechanisms of ZNF831 in oncogenesis remain...Emerging evidence suggested that zinc finger protein 831(ZNF831)was associated with immune activity and stem cell regulation in breast cancer.Whereas,the roles and molec-ular mechanisms of ZNF831 in oncogenesis remain unclear.ZNF831 expression was significantly diminished in breast cancer which was associated with promoter CpG methylation but not mu-tation.Ectopic over-expression of ZNF831 suppressed breast cancer cell proliferation and col-ony formation and promoted apoptosis in vitro,while knockdown of ZNF831 resulted in an opposite phenotype.Anti-proliferation effect of ZNF831 was verified in vivo.Bioinformatic analysis of public databases and transcriptome sequencing both showed that ZNF831 could enhance apoptosis through transcriptional regulation of the JAK/STAT pathway.ChiP and luciferase report assays demonstrated that ZNF831 could directly bind to one specific region of STAT3 promoter and induce the transcriptional inhibition of STAT3.As a result,the attenuation of STAT3 led to a restraint of the transcription of Bcl2 and thus accelerated the apoptotic progression.Augmentation of STAT3 diminished the apoptosis-promoting effect of ZNF831 in breast cancer cell lines.Furthermore,ZNF831 could ameliorate the anti-proliferation effect of capecitabine and gemcitabine in breast cancer cell lines.Our findings demonstrate for the first time that ZNF831 is a novel transcriptional suppressor through inhibiting the expression of STAT3/Bcl2 and promoting the apoptosis process in breast cancer,suggesting ZNF831 as a novel biomarker and potential therapeutic target for breast cancer patients.展开更多
基金National Key R&D Program of China,No.2022YFF1203300.
文摘BACKGROUND Tumor budding(TB)has emerged as a promising independent prognostic biomarker in colorectal cancer(CRC).The prognostic role of TB has been extensively studied and currently affects clinical decision making in patients with stage I and II CRC.However,existing prognostic studies on TB in stage III CRC have been confined to small retrospective cohort studies.Consequently,this study investigated the correlation among TB categories,clinicopathological features,and prognosis in stage III-IV CRC to further enhance the precision and individualization of treatment through refined prognostic risk stratification.AIM To analyze the relationship between TB categories and clinicopathological characteristics and assess their prognostic value in stage III-IV CRC to further refine the prognostic risk stratification of stage III-IV CRC.METHODS The clinical data of 547 CRC patients were collected for this retrospective study.Infiltration at the front edge of the tumor buds was counted according to the 2016 International Tumor Budding Consensus Conference guidelines.RESULTS Multivariate Cox proportional hazards regression analysis demonstrated that chemotherapy(P=0.004),clinical stage IV(P<0.001),≥4 regional lymph node metastases(P=0.004),left-sided colonic cancer(P=0.040),and Bd 2-3(P=0.002)were independent prognostic factors in patients with stage III-IV CRC.Moreover,the density of tumor infiltrating lymphocytes was higher in Bd 1 than in Bd 2-3,both in the tumor stroma and its invasive margin.CONCLUSION TB has an independent predictive prognostic value in patients with stage III-IV CRC.It is recommended to complete the TB report of stage III-IV CRC cases in the standardized pathological report to further refine risk stratification.
基金supported by the National Youth Science Foundation of China(Grant No.52001211).
文摘Target strength(TS)and circular synthetic aperture sonar(CSAS)images provide essential information for active acoustic detection and recognition of non-cooperative unmanned undersea vehicles(UUVs),which pose a significant threat to underwater preset facilities.To access them,we propose an iterative physical acoustics(IPA)-based method to simulate the multiple acoustic scattered fields on rigid surfaces in high-frequency cases.It uses the Helmholtz integral equation with an appropriate Green's function in terms of the Neumann series,and then incorporates the ideas of triangulation and iteration into a numerical implementation.Then two approximate analytic formulae with precise physical meanings are derived to predict the TS and CSAS images of concave targets,respectively.There are no restrictions on the surface's curvature and the order of multiple scattering.The method is validated against the finite element method(FEM)for acoustic scattering from a sphere segment and against an experiment involving an X-rudder UUV's stern.On this basis,we simulate and analyze the TS and CSAS images of an X-rudder UUV.In addition,the influence of the angle of adjacent rudders on the multiple scattering characteristics is discussed.Results show that this method can potentially predict accurate UUV features,especially the multiple scattered features.
基金National Natural Science Foundation of China (51239009)National Science and Technology Support Program of China (2011BAD29B05)+1 种基金Key Discipline Foundation of Water Resources and Hydropower Engineering of Xinjiang Province (XJZDXK-2002-10-05)Natural Science Foundation of Shandong Province (ZR2010EM042)
文摘Optimal use of water and fertilizers can enhance winter wheat yield and increase the efficiencies of water and fertilizer usage in dryland agricultural systems.In order to optimize water and nitrogen(N)management for winter wheat,we conducted field experiments from 2006 to 2008 at the Changwu Agro-ecological Experimental Station of the Chinese Academy of Sciences on the Loess Plateau,China.Regression models of wheat yield and evapotranspiration(ET)were established in this study to evaluate the water and fertilizer coupling effects and to determine the optimal coupling domain.The results showed that there was a positive effect of water and N fertilizer on crop yield,and optimal irrigation and N inputs can significantly increase the yield of winter wheat.In the drought year(2006–2007),the maximum yield(Ymax)of winter wheat was 9.211 t/hm2for the treatment with 324 mm irrigation and 310 kg/hm2N input,and the highest water use efficiency(WUE)of 16.335 kg/(hm2 mm)was achieved with198 mm irrigation and 274 kg/hm2N input.While in the normal year(2007–2008),the maximum winter wheat yield of 10.715 t/hm2was achieved by applying 318 mm irrigation and 291 kg/hm2N,and the highest WUE was 18.69kg/(hm2 mm)with 107 mm irrigation and 256 kg/hm2N input.Crop yield and ET response to irrigation and N inputs followed a quadratic and a line function,respectively.The optimal coupling domain was determined using the elasticity index(EI)and its expression in the water-N dimensions,and was represented by an ellipse,such that the global maximum WUE(WUEmax)and Ymax values corresponded to the left and right end points of the long axis,respectively.Considering the aim to get the greatest profit in practice,the optimal coupling domain was represented by the lower half of the ellipse,with the Ymax and WUEmax on the two end points of the long axis.Overall,we found that the total amount of irrigation for winter wheat should not exceed 324 mm.In addition,our optimal coupling domain visually reflects the optimal range of water and N inputs for the maximum winter wheat yield on the Loess Plateau,and it may also provide a useful reference for identifying appropriate water and N inputs in agricultural applications.
基金supported by National Natural Science Foundation of China under grant Nos. 40839909,41074062, and 40674040
文摘In large continental orogens, an important research topic is the behavior of deep crustal and upper mantle deformation, and the flow styles of ductile material. The morphology of the eastern margin of the Tibetan plateau, adjacent to the Sichuan basin, is characterized by very steep relief with high mountain ranges. The crust beneath this region slows the velocities in the middle and lower crust. We have adopted a relatively dense network to inverse the detailed structure of the crust and upper mantle along the eastern margin of the Tibetan plateau and Sichuan basin, using teleseismic data via receiver function analysis. The results are in-line with the hypothesis that viscous crustal material is flowing beneath the eastern margin of the Tibetan plateau and that this process drives overlying crustal material around the strong and rigid Sichuan basin. When the viscous material hits this obstruction, flows are divided into two or more branches with different directions. The upper part of the upwelling viscous flow produces the pressure to intrude the upper crust, thereby driving uplift of mountain ranges and high peaks. In contrast, the lower part of the downwelling viscous flow produces the pressure to intrude the lower crust and upper mantle to deepen the Moho discontinuity, causing observed crustal thickening.
文摘BACKGROUND: Heart rate variability refers to the beat-to-beat alteration in heart rate. It is usually a slight periodic variation of R-R intervals. Much information of autonomic nerve system balance can be obtained by measuring the heart rate variability of patients. It remains to be shown whether heart rate variability can be used as an index for determining the severity of insomnia and cerebral infarction. OBJECTIVE: This study aimed to analyze the correlation for each frequency spectrum parameter of heart rate variability with an insomnia index, as well as the degree of neurological defects in patients with simple cerebral infarction and cerebral infarction complicated by insomnia. The goal was to verify the feasibility of frequency spectrum parameters for heart rate variability as a marker for insomnia and cerebral infarction. DESIGN: A case-control observation. SETTING: Department of Neurology, First Hospital Affiliated to China Medical University. PARTICIPANTS: Sixty inpatients, and/or outpatients, with cerebral infarction were admitted to the 202 Hospital of Chinese PLA between December 2005 and October 2006, confirmed by CT, and recruited to the study. According to the insomnia condition (insomnia is defined by a Pittsburgh Sleep Quality Index score > 7), the patients were assigned to a simple cerebral infarction group and a cerebral infarction complicated by insomnia group, with 30 subjects in each group. Thirty additional subjects, who concurrently received ex- aminations and were confirmed to not suffer from cerebral infarction and insomnia, were recruited into the control group. Written informed consent was obtained from each subject for laboratory specimens. The pro- tocol was approved by the Hospital's Ethics Committee. METHODS: Following admission, each subject's neurological impairment was assessed with the National Institutes of Health Stroke Scale and Pittsburgh Sleep Quality Index. Heart rate variability of each subject was measured with an autonomic nerve analyzer (Weijin Science and Technology Co., Ltd., Taiwan). Each frequency spectrum parameter of heart rate variability was obtained, including very low frequency, low fre- quency, high frequency, total power, R-R interval, and its mean square. In addition, percentage of low fre- quency, high frequency, and ratio of low frequency to high frequency were calculated. For each heart rate frequency spectrum parameter, the difference between groups was analyzed. Moreover, correlations of each frequency spectrum parameter with insomnia and disease condition were analyzed. Data from each index, which were not normally distributed, were processed by logarithmic transformation. The t-test was used for the comparison of intergroup differences. Single-factor linear regression analysis and t-test were used for the analysis of factor-factor correlation and coefficient of correlation, respectively. MAIN OUTCOME MEASURES: ① Differences of scores in the National Institutes of Health Stroke Scale and Pittsburgh Sleep Quality Index between the simple cerebral infarction group and the cerebral in- farction complicated by insomnia group. ② Differences of heart rate variability parameters between the simple cerebral infarction group and the control group. ③ Correlation of heart rate variability parameters, the Pittsburgh Sleep Quality Index score, and the neurological impairment score. RESULTS: Sixty patients and thirty healthy controls were included in the final analysis. ① The scores of the Pittsburgh Sleep Quality Index and the neurological impairment were significantly higher in the cerebral infarction complicated by insomnia group compared to the simple cerebral infarction group (P < 0.05-0.01). ② R-R interval was significantly longer in the simple cerebral infarction group than in the control group, while R-R interval variance and high-frequency band were significantly lower in the simple cerebral infarc- tion group compared to the control group (P < 0.05). ③ For cerebral infarction patients with insomnia, the Pittsburgh Sleep Quality Index score was significantly positively correlated with neurological impairment (r = 0.54, P < 0.01). The low-frequency band, very low-frequency band, high-frequency band, R-R interval variance, total power, R-R interval, and the percentage of high-frequency were significantly negativelycorrelated with the National Institutes of Health Stroke Scale score (r =-0.45 to -0.90, P < 0.05-0.01) and with the Pittsburgh Sleep Quality Index scores (r = -0.56 to -0.36, P < 0.05-0.01). CONCLUSION: Each heart rate variability parameter can be used as an index for assessing dyssomnia and neurologic impairment (r =-0.56 to -0.36, P < 0.05-0.01).
基金the National Natural Science Foundation of China(No.81472707)Chinese South Western Oncology Group(CSWOG-CCET005).
文摘The incidence of colorectal cancer(CRC)is increasing in China,with high mortality.Here,we aimed to evaluate the latest clinicopathological features and prognostic value of the KRAS/NRAS/BRAF mutation status in CRC patients in Central China.The clinical data of 1549 CRC patients with stage I-IV disease diagnosed at Union Hospital,Tongji Medical College of Huazhong University of Science and Technology from 2015 to 2017 were collected and analyzed retrospectively.KRAS/NRAS/BRAF mutations were detected by real-time quantitative polymerase chain reaction(q-PCR)in 410 CRC patients,with mutation frequencies of KRAS,NRAS and BRAF of 47.56%,2.93%and 4.15%,respectively.The gene mutation status and clinicopathological characteristics of 410 patients with CRC who underwent qPCR were analyzed.The KRAS and BRAF gene mutations were related to the pathological differentiation and number of metastatic lymph nodes.The BRAF gene mutation was also associated with cancer thrombosis in blood vessels.Cox regression analysis showed that there was no statistically significant difference in the overall survival(OS)between patients with KRAS,NRAS mutants and wild-type CRC patients,while the BRAF gene mutation was negatively correlated with the OS rate of CRC patients.It is suggested that the BRAF gene mutation may be an independent risk factor for the prognosis of CRC.
基金the Oceanic Interdisciplinary Program of Shanghai Jiao Tong University(project number SL2021PT108)。
文摘A numerical triangulation and transformation into the time domain of a Kirchhoff approximation(KA)method is proposed for the modeling of bistatic scattering from an underwater non-penetrable target.The time domain solution in this approximation can be split up into two parts:the solution of reflected field,contributing around the specular direction,and the solution of shadow radiation,contributing around the forward direction.An average solution in the time domain satisfying the reciprocity principle is presented.The solution is expressed in terms of non-singular functions.The proposed method is validated against a normal mode method for bistatic scattering from a rigid sphere.Moreover,the reflected and shadow highlights on the surface of the sphere are shown to verify the integration surface of the reflected field and shadow radiation.It is also tested against a finite element method and an experiment involving a scaled Benchmark Target Strength Simulation Submarine model.The time-angle bistatic spectra for the model are evaluated by the direct and transformed average solutions of KA,and the former accelerates its speed of calculation.The results are good,and show that this method can be used to predict the bistatic scattered field of a non-penetrable target.
文摘A new acrylic ester polymer YWB-7 resin was prepared and characterized. The properties of YWB-7 resin were compared with those of the commercial Amberlite XAD-7, Diaion HP2MG and hypercrosslinked macroporous polymer NDA-150 resins. Both surface area and micropore area of YWB-7 resin were bigger than those of XAD-7 resin and HP2MG resin. The YWB-7 resin was successfully employed to recycle 5-sodiosulfoisophthalic acids (SIPA) from its solutions with and without methanol.
文摘Dissipation kinetics of chlorpyrifos in the near-neutral paddy soils of a vegetable cropping system under greenhouse, screenhouse and field conditions was studied using a rapid analytical method. The recoveries of chlorpyrifos were between 86.5% and 105.5% with relative standard deviations for repeatability between 6.6% and 9.1% at fortification levels of 0.01, 0.1 and 1 mg/kg in the soil. The limit of detection of the method was 0.004 mg/kg and the limit of quantification was found to be 0.01 mg/kg. The dissipation rates of chlorpyrifos followed the first-order kinetics and the half-life was 6.96, 6.04 and 5.20 days in the soil under greenhouse, screenhouse and field conditions, respectively. The dissipation rates of chlorpyrifos in the soil varied with different cultivation conditions. Chlorpyrifos in the soil dissipated slower in a greenhouse and screenhouse than in the open field, which was likely attributed to the hermetic environment in the greenhouse and screenhouse.
基金the National Key Research and Development Program of China(2016YFA0601003)。
文摘Organic carbon(OC)source attribution for cascade reservoir sediments has been identified as a critical gap in understanding the effective carbon sink of inland waters.In this study,nine sediment cores were collected from cascade reservoirs in the Wujiang and Pearl Rivers.We analyzed lignin phenol(Λ8),total organic carbon(TOC)content,and stable carbon isotopic composition(δC)of the sediments,focusing on the changes of terrigenous OC,and the variation of OC source in cascade reservoir sediments after damming.Our results showed that theΣ8 and TOC contents decreased from upstream to downstream reservoirs,indicating the significant interception of terrigenous OC by cascade damming.Additionally,theΛ8 content in the Pearl River reservoir sediments was much higher than that in the Wujiang River.From the three-end-member mixing model,we estimated that OC in reservoir sediments mainly comes from soil and plankton.After damming,the proportion of plankton OC in TOC slightly increased in seasonal and annual regulation reservoirs due to the limnetic evolution of the reservoir.These findings suggest that the cascade damming increases the interception capacity of the river to terrigenous OC and nutrients,and that slowing of water velocity caused by damming affected primary productivity and fluvial carbon cycling.
文摘Objective: To investigate human cytomegalovirus infec-tion and genetic variations in glycoprotein B(gB) inliver transplant recipients in south-east China.Methods:EDTA-blood samples were obtained from 21liver transplant recipients. The semi-nested PCR wasused to amplify a region of high sequence variabilityin the gB gene of human cytomegalovirus (HCMV)followed by direct sequence analysis.Results: Out of the 21 liver transplant recipients, 5were proved HCMV positive 62 to 180 days aftertransplantation. The nucleotide and encoded aminoacid sequences were compared with published se-quences of AD169 and Towne laboratory strains.Within the region sequenced, 2 out of 5 strains pos-sessed a peptide configuration similar to that of strainAD169, while another 2 strains displayed a peptideconfiguration similar to that of strain Towne. Onestrain had amino acid substitution, which was differ-ent from those of both AD169 and Towne in thecleavage site.Conclusion: Our results provide molecular epidemio-logical data for HCMV strains circulating among trans-plant recipients in south-east China.
文摘Objective: To explore cytomegalovirus (CMV) infec-tion in recipients of liver transplantation (LT).Methods: 30 recipients of LT were screened for theappearance of CMV infection by using ELISA to testanti-CMV-Ab from serum samples and using im-munohistochemistry method to test CMV antigen ex-pression and nested-PCR to amplify CMV-DNA fromblood samples.Results: Four of 243 samples taken from 30 recipientscame out positive of anti-CMV IgC; and anti-CMVIgM with a positive rate of 100% and 1.6% respec-tively. 85 samples resulted in CMV antigen expression(35.0%) with the average antigen index being 4.2±3.1/5×10~4 WBC. Besides, 99 samples were found tobe positive by nested-PCR with a positive rate of40.7%. 61 samples were found to be simultaneously pos-itive in test of CMV antigen and DNA, with a rate of25.1%.Conclusions: Infection of CMV is common in recipi-ents of LT. Simultaneous screening of anti-CMV-Ab, CMV-Ag and CMV-DNA after liver transplanta-tion is very important for early diagnosis of CMV in-fection.
基金supported by the National Natural Science Foundation of China(No.81472482,82173556)the Clinical Technology Innovation and Cultivation Project of the Army Medical University of China(No.CX2019LC120)the National Key Clinical Specialty Military Construction Project(China,No.425Z8).
文摘Emerging evidence suggested that zinc finger protein 831(ZNF831)was associated with immune activity and stem cell regulation in breast cancer.Whereas,the roles and molec-ular mechanisms of ZNF831 in oncogenesis remain unclear.ZNF831 expression was significantly diminished in breast cancer which was associated with promoter CpG methylation but not mu-tation.Ectopic over-expression of ZNF831 suppressed breast cancer cell proliferation and col-ony formation and promoted apoptosis in vitro,while knockdown of ZNF831 resulted in an opposite phenotype.Anti-proliferation effect of ZNF831 was verified in vivo.Bioinformatic analysis of public databases and transcriptome sequencing both showed that ZNF831 could enhance apoptosis through transcriptional regulation of the JAK/STAT pathway.ChiP and luciferase report assays demonstrated that ZNF831 could directly bind to one specific region of STAT3 promoter and induce the transcriptional inhibition of STAT3.As a result,the attenuation of STAT3 led to a restraint of the transcription of Bcl2 and thus accelerated the apoptotic progression.Augmentation of STAT3 diminished the apoptosis-promoting effect of ZNF831 in breast cancer cell lines.Furthermore,ZNF831 could ameliorate the anti-proliferation effect of capecitabine and gemcitabine in breast cancer cell lines.Our findings demonstrate for the first time that ZNF831 is a novel transcriptional suppressor through inhibiting the expression of STAT3/Bcl2 and promoting the apoptosis process in breast cancer,suggesting ZNF831 as a novel biomarker and potential therapeutic target for breast cancer patients.
