The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance.However,the genetic basis of muscle development in the Boer goat remains ...The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance.However,the genetic basis of muscle development in the Boer goat remains obscure.In this study,we identified specific structural variants in the Boer goat based on genome-wide selection signals and analyzed the basis of the molecular heredity of related candidate genes in muscle development.A total of9 959 autosomal copy number variations(CNVs) were identified through selection signal analysis in 127 goat genomes.Specifically,we confirmed that the highest signal CNV(HSV) was a chromosomal arrangement containing an approximately 1.11 Mb(CHIR17:60062304-61171840 bp) duplicated fragment inserted in reverse orientation and a 5 362 bp deleted region(CHIR17:60145940-60151302 bp) with overlapping genes(e.g.,ARHGAP10,NR3C2,EDNRA,PRMT9,and TMEM184C).The homozygous duplicated HSV genotype(+/+) was found in 96% of Boer goats but was not detected in Eurasian goats and was only detected in 4% of indigenous African goats.The expression network of three candidate genes(ARHGAP10,NR3C2,and EDNRA)regulating dose transcription was constructed by RNA sequencing.Results indicated that these genes were involved in the proliferation and differentiation of skeletal muscle satellite cells(SMSCs) and their overexpression significantly increased the expression of SAA3.The HSV of the Boer goat contributed to superior skeletal muscle growth via the dose effects of overlapping genes.展开更多
As the only working dog independently developed and bred in China,Chinese Kunming dog(CKD)was approved as a new breed by the Chinese National Livestock and Poultry Genetics Commission in 2007.Now CKD is widely used by...As the only working dog independently developed and bred in China,Chinese Kunming dog(CKD)was approved as a new breed by the Chinese National Livestock and Poultry Genetics Commission in 2007.Now CKD is widely used by the Chinese army and police and has been exported to Singapore,Thailand,Vietnam,North Korea,and other more than 10 countries as working dog.Previously published studies of CKD have focused on coat color genetics(Wang et al.2013)and reproduction performance(Wei et al.2018).The study by Wang et al.(2013)pointed out that the breed of CKD was developed in the 1950s by hybridizing German shepherd dogs(GSDs)imported from the Soviet Union with Kunming indigenous village dogs.展开更多
基金supported by the National Natural Science Foundation of China (32272834)。
文摘The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance.However,the genetic basis of muscle development in the Boer goat remains obscure.In this study,we identified specific structural variants in the Boer goat based on genome-wide selection signals and analyzed the basis of the molecular heredity of related candidate genes in muscle development.A total of9 959 autosomal copy number variations(CNVs) were identified through selection signal analysis in 127 goat genomes.Specifically,we confirmed that the highest signal CNV(HSV) was a chromosomal arrangement containing an approximately 1.11 Mb(CHIR17:60062304-61171840 bp) duplicated fragment inserted in reverse orientation and a 5 362 bp deleted region(CHIR17:60145940-60151302 bp) with overlapping genes(e.g.,ARHGAP10,NR3C2,EDNRA,PRMT9,and TMEM184C).The homozygous duplicated HSV genotype(+/+) was found in 96% of Boer goats but was not detected in Eurasian goats and was only detected in 4% of indigenous African goats.The expression network of three candidate genes(ARHGAP10,NR3C2,and EDNRA)regulating dose transcription was constructed by RNA sequencing.Results indicated that these genes were involved in the proliferation and differentiation of skeletal muscle satellite cells(SMSCs) and their overexpression significantly increased the expression of SAA3.The HSV of the Boer goat contributed to superior skeletal muscle growth via the dose effects of overlapping genes.
基金supported by the National Key R&D Program of China(2019YFA0707101)Key Research Program of Frontier Sciences of the CAS(ZDBS-LY-SM011)+1 种基金Innovative Research Team(in Science and Technology)of Yunnan Province(201905E160019)G.-D.W.is supported by the Youth Innovation Promotion Association of CAS.
文摘As the only working dog independently developed and bred in China,Chinese Kunming dog(CKD)was approved as a new breed by the Chinese National Livestock and Poultry Genetics Commission in 2007.Now CKD is widely used by the Chinese army and police and has been exported to Singapore,Thailand,Vietnam,North Korea,and other more than 10 countries as working dog.Previously published studies of CKD have focused on coat color genetics(Wang et al.2013)and reproduction performance(Wei et al.2018).The study by Wang et al.(2013)pointed out that the breed of CKD was developed in the 1950s by hybridizing German shepherd dogs(GSDs)imported from the Soviet Union with Kunming indigenous village dogs.
