The first intron of human hsp 90β gene is not only essential m maintaining high constitutive expression but also critical for heat shock inducibility of the hsp90β gene. Typical HSEs in the first intron play a vital...The first intron of human hsp 90β gene is not only essential m maintaining high constitutive expression but also critical for heat shock inducibility of the hsp90β gene. Typical HSEs in the first intron play a vital role in the heat induced expression of human hsp90β gene. Slot blot analysis shows that hsp90β gene mRNA transcripts initiated from the 3'' of the first intron dominates over that of the first exon. The intronic HSEs of the hsp90β gene show much higher binding affinity toward the recombinant heat shock factor HSF1 than that of the recombinant heat shock factor HSF2.展开更多
Heat shock proteins (HSP) are a group of proteins highly conserved in evolution.They play important physiological roles, usually as molecular chaperones in living cells, and take great parts in cell protection process...Heat shock proteins (HSP) are a group of proteins highly conserved in evolution.They play important physiological roles, usually as molecular chaperones in living cells, and take great parts in cell protection process against stress, and in signal transduction as well.HSP70 and HSP90 are the major HSPs expressed in human peripheral lymphocytes upon heat shock. HSP90 consists of two copies, designated as HSP90α and HSP90β. Heat shock factors (HSF), the trans regulators, participate in the regulation of HSP gene transcription. Different from HSP70 which is dramatically induced by stress, both heat shock and cold shock, HSP90 expresses at a relatively high level physiologically and may exist as complexes with various steroid receptors or some important components in signal transduction pathway, and plays a role of specific molecular chaperone. The aim of展开更多
文摘The first intron of human hsp 90β gene is not only essential m maintaining high constitutive expression but also critical for heat shock inducibility of the hsp90β gene. Typical HSEs in the first intron play a vital role in the heat induced expression of human hsp90β gene. Slot blot analysis shows that hsp90β gene mRNA transcripts initiated from the 3'' of the first intron dominates over that of the first exon. The intronic HSEs of the hsp90β gene show much higher binding affinity toward the recombinant heat shock factor HSF1 than that of the recombinant heat shock factor HSF2.
基金Project supported by the National Natural Science Foundation of Chinathe Ministry of Health of China.
文摘Heat shock proteins (HSP) are a group of proteins highly conserved in evolution.They play important physiological roles, usually as molecular chaperones in living cells, and take great parts in cell protection process against stress, and in signal transduction as well.HSP70 and HSP90 are the major HSPs expressed in human peripheral lymphocytes upon heat shock. HSP90 consists of two copies, designated as HSP90α and HSP90β. Heat shock factors (HSF), the trans regulators, participate in the regulation of HSP gene transcription. Different from HSP70 which is dramatically induced by stress, both heat shock and cold shock, HSP90 expresses at a relatively high level physiologically and may exist as complexes with various steroid receptors or some important components in signal transduction pathway, and plays a role of specific molecular chaperone. The aim of
