From October 2006,we began to investigate two kinds of mild stimulation protocols which were CC+HMG and L+HMG.Our preliminary results show:(1)119 patients were stimulated with CC+HMG,143 oocyte pick-up(OPU) cycles hav...From October 2006,we began to investigate two kinds of mild stimulation protocols which were CC+HMG and L+HMG.Our preliminary results show:(1)119 patients were stimulated with CC+HMG,143 oocyte pick-up(OPU) cycles have been finished.The average age of patient was 33.9 years old.5.64 eggs were retrieved per patient averagely.Fertilization rate was 63.4%.The 143 OPU cycles resulted in 59 fresh embryo transfer cycles(11 clinical pregnancies),55 whole embryo frozen cycles and 29 cycles without available embryo(15 cycles were due to un-fertilization or un-cleavage,14 cycles were due to blastocyst culture failure).In 85 frozen embryo transfer cycles,the clinical pregnancy rate was 20%(17/85).Until now,3 patients of whole embryo frozen cycle still haven’t carried out frozen embryo transfer.After transfer,3 patients without pregnancy still have frozen embryos.We anticipate that there will be one pregnancy at most in these 3 patients.Therefore,the cumulative pregnancy rate will achieve 20.7%(29/440) in 140 finished OPU cycles.No moderate or severe OHSS happened.(2)From July 2007 to April 2008,we had finished 284 OPU cycles with L+HMG protocols.The 284 OPU cycles resulted in 261 fresh embryo transfer cycles(64 clinical pregnancies),10 whole embryo frozen cycles and 13 cycles without available embryo.Clinical pregnancy rate was 24.5% for fresh embryo transfer cycles.Excluding 10 whole embryo frozen cycles,the clinical pregnancy rate for OPU cycles was 23.4(64/274).No moderate or severe OHSS happened in those 284 OPU cycles.Until 10,April 2008,72 thawed transfer cycles had been finished for above cases,20 pregnancies were produced and the pregnancy rate was 27.8%.The experiences of 427 mild stimulation cycles demonstrate that CC+HMG or L+HMG protocol show unique benefits,especially for patients with repeated IVF-ET failure,empty follicle syndrome,fertilization failure in COH cycles,poor-respond and hyper-respond to COH.展开更多
Connexins and pannexins are two protein families that play an important role in cellular communication. Pannexin 1 (PANX1), one of the members of pannexin family, is a channel protein. It is glycosylated and forms thr...Connexins and pannexins are two protein families that play an important role in cellular communication. Pannexin 1 (PANX1), one of the members of pannexin family, is a channel protein. It is glycosylated and forms three species, GLY0, GLY1, and GLY2. Here, we describe four independent families in which mutations in PANX1 cause familial or sporadic female infertility via a phenotype that we term “oocyte death.” The mutations, which are associated with oocyte death, alter the PANX1 glycosylation pattern, influence the subcellular localization of PANX1 in cultured cells, and result in aberrant PANX1 channel activity, ATP release in oocytes, and mutant PANX1 GLY1. Overexpression of a patient-derived mutation in mice causes infertility, recapitulating the human oocyte death phenotype. Our findings demonstrate the critical role of PANX1 in human oocyte development, provide a genetic explanation for a subtype of infertility, and suggest a potential target for therapeutic intervention for this disease.展开更多
目的探讨中医(补肾健脾)预培其损序贯疗法对胚胎反复移植失败(repeated implantation fail-ure,RIF)患者新鲜周期相关指标的影响。方法将既往行体外受精-胚胎移植(in virto fertilization and em-bryo transfer,IVF-ET)或卵母细胞单精...目的探讨中医(补肾健脾)预培其损序贯疗法对胚胎反复移植失败(repeated implantation fail-ure,RIF)患者新鲜周期相关指标的影响。方法将既往行体外受精-胚胎移植(in virto fertilization and em-bryo transfer,IVF-ET)或卵母细胞单精子显微注射(intracytoplasmic sperm injection,ICSI)失败3次及以上的患者,行新鲜取卵/移植周期共60例,分为治疗组(中药结合IVF/ICSI)与对照组(单纯IVF/ICSI),每组30例,比较两组外源性促性腺激素(gonadotropin,Gn)用量、超排卵(controlled ovarian hyperstimulation,COH)时间、移植日子宫内膜厚度、获得卵数、受精数、胚胎数、优质胚胎数、妊娠率及种植率等各项指标的差异。结果治疗组单周期人均获得胚胎(7.5±4.9)个,优质胚胎(5.1±3.8)个,两者较对照组(5.1±3.2,3.2±1.8)均明显升高,差异均有统计学意义(P<0.05)。两组在Gn用量、COH天数、移植日子宫内膜厚度、获得卵数、受精数、妊娠率及种植率方面比较,差异均无统计学意义(P>0.05)。结论中医预培其损序贯疗法结合超排卵有利于胚胎数量和质量的双重提高,有望增加RIF患者的累计妊娠率。展开更多
文摘From October 2006,we began to investigate two kinds of mild stimulation protocols which were CC+HMG and L+HMG.Our preliminary results show:(1)119 patients were stimulated with CC+HMG,143 oocyte pick-up(OPU) cycles have been finished.The average age of patient was 33.9 years old.5.64 eggs were retrieved per patient averagely.Fertilization rate was 63.4%.The 143 OPU cycles resulted in 59 fresh embryo transfer cycles(11 clinical pregnancies),55 whole embryo frozen cycles and 29 cycles without available embryo(15 cycles were due to un-fertilization or un-cleavage,14 cycles were due to blastocyst culture failure).In 85 frozen embryo transfer cycles,the clinical pregnancy rate was 20%(17/85).Until now,3 patients of whole embryo frozen cycle still haven’t carried out frozen embryo transfer.After transfer,3 patients without pregnancy still have frozen embryos.We anticipate that there will be one pregnancy at most in these 3 patients.Therefore,the cumulative pregnancy rate will achieve 20.7%(29/440) in 140 finished OPU cycles.No moderate or severe OHSS happened.(2)From July 2007 to April 2008,we had finished 284 OPU cycles with L+HMG protocols.The 284 OPU cycles resulted in 261 fresh embryo transfer cycles(64 clinical pregnancies),10 whole embryo frozen cycles and 13 cycles without available embryo.Clinical pregnancy rate was 24.5% for fresh embryo transfer cycles.Excluding 10 whole embryo frozen cycles,the clinical pregnancy rate for OPU cycles was 23.4(64/274).No moderate or severe OHSS happened in those 284 OPU cycles.Until 10,April 2008,72 thawed transfer cycles had been finished for above cases,20 pregnancies were produced and the pregnancy rate was 27.8%.The experiences of 427 mild stimulation cycles demonstrate that CC+HMG or L+HMG protocol show unique benefits,especially for patients with repeated IVF-ET failure,empty follicle syndrome,fertilization failure in COH cycles,poor-respond and hyper-respond to COH.
文摘Connexins and pannexins are two protein families that play an important role in cellular communication. Pannexin 1 (PANX1), one of the members of pannexin family, is a channel protein. It is glycosylated and forms three species, GLY0, GLY1, and GLY2. Here, we describe four independent families in which mutations in PANX1 cause familial or sporadic female infertility via a phenotype that we term “oocyte death.” The mutations, which are associated with oocyte death, alter the PANX1 glycosylation pattern, influence the subcellular localization of PANX1 in cultured cells, and result in aberrant PANX1 channel activity, ATP release in oocytes, and mutant PANX1 GLY1. Overexpression of a patient-derived mutation in mice causes infertility, recapitulating the human oocyte death phenotype. Our findings demonstrate the critical role of PANX1 in human oocyte development, provide a genetic explanation for a subtype of infertility, and suggest a potential target for therapeutic intervention for this disease.
