To achieve better cycling performance and vibration comfort of mountain bicycle, the optimization of frame structural parameters and rear suspension scale parameters is investigated based on biomechanics.Firstly, the ...To achieve better cycling performance and vibration comfort of mountain bicycle, the optimization of frame structural parameters and rear suspension scale parameters is investigated based on biomechanics.Firstly, the quadratic sum of rider lower limb muscles stresses is presented as the evaluation criterion of muscle fatigue.By taking the criterion as the objective function, the relative positions of three pivot points of frame are optimized to ensure that the frame structural parameters match the stature o...展开更多
为了明确我国犬瘟热病毒流行毒株的关键毒力基因及其致病机制,本研究构建了一株表达疫苗毒株H基因的嵌合犬瘟热病毒rHBF-vacH。犬瘟热病毒的囊膜蛋白H和F,决定着犬瘟热病毒宿主嗜性和致病性,也是产生免疫保护的关键抗原。因此,本研究以G...为了明确我国犬瘟热病毒流行毒株的关键毒力基因及其致病机制,本研究构建了一株表达疫苗毒株H基因的嵌合犬瘟热病毒rHBF-vacH。犬瘟热病毒的囊膜蛋白H和F,决定着犬瘟热病毒宿主嗜性和致病性,也是产生免疫保护的关键抗原。因此,本研究以GenBank中公布的犬瘟热病毒疫苗毒株Onderstepoort序列(AF378705)为模板,利用Primer Premier 6.0设计特异性引物,扩增其H基因,将Onderstepoort的H基因定向克隆至pcDNA3.2-HBF-1中,筛选获得正确的全长重组质粒pcDNA3.2-HBF-vacH之后,通过病毒拯救,获得嵌合病毒rHBF-vacH,最后,通过免疫荧光、RT-PCR和电镜观察,初步鉴定嵌合病毒rHBF-vacH的基因组中携带有犬瘟热病毒疫苗毒Onderstepoort的H基因。本研究构建的嵌合病毒rHBF-vacH为国内犬瘟热病毒流行毒株H基因致病性研究提供了技术平台,有助于设计重组犬瘟热疫苗。展开更多
基金Supported by Tianjin Science and Technology Development Project (No.043186211)Tianjin Key Laboratory of Advanced Manufacturing Technology and Equipment
文摘To achieve better cycling performance and vibration comfort of mountain bicycle, the optimization of frame structural parameters and rear suspension scale parameters is investigated based on biomechanics.Firstly, the quadratic sum of rider lower limb muscles stresses is presented as the evaluation criterion of muscle fatigue.By taking the criterion as the objective function, the relative positions of three pivot points of frame are optimized to ensure that the frame structural parameters match the stature o...
文摘为了明确我国犬瘟热病毒流行毒株的关键毒力基因及其致病机制,本研究构建了一株表达疫苗毒株H基因的嵌合犬瘟热病毒rHBF-vacH。犬瘟热病毒的囊膜蛋白H和F,决定着犬瘟热病毒宿主嗜性和致病性,也是产生免疫保护的关键抗原。因此,本研究以GenBank中公布的犬瘟热病毒疫苗毒株Onderstepoort序列(AF378705)为模板,利用Primer Premier 6.0设计特异性引物,扩增其H基因,将Onderstepoort的H基因定向克隆至pcDNA3.2-HBF-1中,筛选获得正确的全长重组质粒pcDNA3.2-HBF-vacH之后,通过病毒拯救,获得嵌合病毒rHBF-vacH,最后,通过免疫荧光、RT-PCR和电镜观察,初步鉴定嵌合病毒rHBF-vacH的基因组中携带有犬瘟热病毒疫苗毒Onderstepoort的H基因。本研究构建的嵌合病毒rHBF-vacH为国内犬瘟热病毒流行毒株H基因致病性研究提供了技术平台,有助于设计重组犬瘟热疫苗。
