An effective and fast method for assay of micro-amounts of glucose was set up. A new technique for preparation of enzyme columns based on enzyme immobilization by sol-gel was investigated. Glucose oxidase(GOD) and hor...An effective and fast method for assay of micro-amounts of glucose was set up. A new technique for preparation of enzyme columns based on enzyme immobilization by sol-gel was investigated. Glucose oxidase(GOD) and horseradish peroxidase(HRP) mixed with SiO 2 nanoparticles and polyvinyl butyral(PVB) medium were immobilized on the surface of capillary tube, respectively. The experimental results show that nanoparticles can significantly enhance the catalytic activity of immobilized enzyme. Based on GOD column and HRP column, a liquid droplet sensor was developed for the determination of glucose solution. The effect of separated columns and mixed columns on the response of glucose sensor was investigated. The sensor showed a linear response in a range of 2-400 ng/mL with a detection limit of 0.3 ng/mL under the optimum conditions. The characteristics of the sensor including effect of flow-rate, pH and temperature were discussed.展开更多
目的总结肿瘤患者外周静脉置入中心静脉导管(peripherally inserted central catheter,PICC)并发症管理与质量控制的方法。方法对2008年11月2010年3月收治的398例置入PICC的肿瘤患者,采用建立专业化的PICC治疗团队,设计PICC置管临床路径...目的总结肿瘤患者外周静脉置入中心静脉导管(peripherally inserted central catheter,PICC)并发症管理与质量控制的方法。方法对2008年11月2010年3月收治的398例置入PICC的肿瘤患者,采用建立专业化的PICC治疗团队,设计PICC置管临床路径,专业护士置管操作,对常见PICC并发症实施规范化的质量监控与管理。结果 398例PICC治疗患者发生导管相关并发症37例,总发生率为9.3%,其导管堵塞、静脉血栓、机械性静脉炎、导管相关性血流感染等并发症均有发生,但发生率均控制在较低范围。结论重视和规范PICC的操作与维护管理,可最大限度降低PICC相关并发症,确保PICC置管患者的治疗安全。展开更多
文摘An effective and fast method for assay of micro-amounts of glucose was set up. A new technique for preparation of enzyme columns based on enzyme immobilization by sol-gel was investigated. Glucose oxidase(GOD) and horseradish peroxidase(HRP) mixed with SiO 2 nanoparticles and polyvinyl butyral(PVB) medium were immobilized on the surface of capillary tube, respectively. The experimental results show that nanoparticles can significantly enhance the catalytic activity of immobilized enzyme. Based on GOD column and HRP column, a liquid droplet sensor was developed for the determination of glucose solution. The effect of separated columns and mixed columns on the response of glucose sensor was investigated. The sensor showed a linear response in a range of 2-400 ng/mL with a detection limit of 0.3 ng/mL under the optimum conditions. The characteristics of the sensor including effect of flow-rate, pH and temperature were discussed.
文摘目的总结肿瘤患者外周静脉置入中心静脉导管(peripherally inserted central catheter,PICC)并发症管理与质量控制的方法。方法对2008年11月2010年3月收治的398例置入PICC的肿瘤患者,采用建立专业化的PICC治疗团队,设计PICC置管临床路径,专业护士置管操作,对常见PICC并发症实施规范化的质量监控与管理。结果 398例PICC治疗患者发生导管相关并发症37例,总发生率为9.3%,其导管堵塞、静脉血栓、机械性静脉炎、导管相关性血流感染等并发症均有发生,但发生率均控制在较低范围。结论重视和规范PICC的操作与维护管理,可最大限度降低PICC相关并发症,确保PICC置管患者的治疗安全。
