Objective: Peroxisome proliferator-activated receptor -γ(PPAR-γ) plays a critical role in adipocyte differentiation and the development of type 2 diabetes mellitus (T2DM). Numerous studies across several populations...Objective: Peroxisome proliferator-activated receptor -γ(PPAR-γ) plays a critical role in adipocyte differentiation and the development of type 2 diabetes mellitus (T2DM). Numerous studies across several populations have indicated that Pro12Ala polymorphism of PPAR-γ is associated with decreased insulin resistance and decreased risk of T2DM. The aims of this study are to develop a simple and sensitive detection of Pro12Ala polymorphism and examined the distribution of this polymorphism in Chinese population. Methods: The PPAR-γ gene fragment containing Pro12Ala variant of 101 T2DM patients and 104 controls were amplified by PCR amplification and the extension reaction was performed using primer that adjacent to the single nucleotide polymorphic site in presence of two different dye-labeled terminators. The primer's specially extending reactions make the increase of their fluorescence polarization (FP) that mean special genotype. The variant frequencies of the two groups were compared. Results: We detected the Pro12Ala variant successfully by TDI-FP method and we found no significant association between this polymorphism and T2DM in case-control study. Conclusion: The TDI-FP technology is a new specific and sensitive method that is suitable for automatic detection of large number of clinical samples. Prol2Ala mutation in PPAR--@2 gene does not play a significant role in T2DM risk in Chinese population.展开更多
文摘Objective: Peroxisome proliferator-activated receptor -γ(PPAR-γ) plays a critical role in adipocyte differentiation and the development of type 2 diabetes mellitus (T2DM). Numerous studies across several populations have indicated that Pro12Ala polymorphism of PPAR-γ is associated with decreased insulin resistance and decreased risk of T2DM. The aims of this study are to develop a simple and sensitive detection of Pro12Ala polymorphism and examined the distribution of this polymorphism in Chinese population. Methods: The PPAR-γ gene fragment containing Pro12Ala variant of 101 T2DM patients and 104 controls were amplified by PCR amplification and the extension reaction was performed using primer that adjacent to the single nucleotide polymorphic site in presence of two different dye-labeled terminators. The primer's specially extending reactions make the increase of their fluorescence polarization (FP) that mean special genotype. The variant frequencies of the two groups were compared. Results: We detected the Pro12Ala variant successfully by TDI-FP method and we found no significant association between this polymorphism and T2DM in case-control study. Conclusion: The TDI-FP technology is a new specific and sensitive method that is suitable for automatic detection of large number of clinical samples. Prol2Ala mutation in PPAR--@2 gene does not play a significant role in T2DM risk in Chinese population.
文摘目的 观察转录因子ZBTB18对结直肠癌细胞的生长的影响。方法 从NCBI GEO数据库中下载肿瘤基因表达芯片数据,对ZBTB18基因的表达水平进行分析;同时通过RNA干扰和Transwell实验研究ZBTB18对结直肠癌细胞迁移的影响。结果 ZBTB18蛋白表达于结直肠癌细胞系,且高转移能力的细胞系中该蛋白的表达水平远高于原发性结直肠癌肿瘤细胞系( P <0.05)。RNA干扰试验可特异性地显著沉默结直肠癌细胞HT29中ZBTB18的表达,Transwell实验发现在ZBTB18沉默后HT29细胞的侵袭和迁移能力显著降低( P <0.05)。结论 转录因子ZBTB18可以促进结直肠癌细胞的侵袭与迁移,可能参与结直肠癌的转移。
