目的研究乙酰唑胺对培养的牛角膜内皮细胞液体转运功能的影响。方法观察并记录给予不同浓度乙酰唑胺(0.1μmol/L,1μmol/L,10μmol/L,100μmol/L)后角膜内皮细胞在低渗溶液中的体积改变,并计算其渗透水通透性(osmotic water permeabilit...目的研究乙酰唑胺对培养的牛角膜内皮细胞液体转运功能的影响。方法观察并记录给予不同浓度乙酰唑胺(0.1μmol/L,1μmol/L,10μmol/L,100μmol/L)后角膜内皮细胞在低渗溶液中的体积改变,并计算其渗透水通透性(osmotic water permeability,Pf)的变化。结果培养的正常牛角膜内皮细胞的Pf值为(0.0446±0.0089)cm/s,在0.1～100μmol/L乙酰唑胺孵育72h后,培养的牛角膜内皮细胞的渗透性水通透性逐渐减少,其Pf值分别为(0.0406±0.0014)cm/s,(0.0364±0.0015)cm/s,(0.0300±0.0022)cm/s,(0.0226±0.0019)cm/s。结论乙酰唑胺呈剂量依赖性地减低角膜内皮渗透性水通透性,抑制角膜内皮细胞液体转运功能。展开更多
The expression of VEGF-C and molecular mechanisms of lymphangiogenesis in rat cornea after alkali injury was studied. The rat alkali injured corneal models were made. Under electron microscopy, the lymphatic vessels i...The expression of VEGF-C and molecular mechanisms of lymphangiogenesis in rat cornea after alkali injury was studied. The rat alkali injured corneal models were made. Under electron microscopy, the lymphatic vessels in the rat injured corneas were examined. The expression of VEGF-C proteins was detected by using immunohistochemical assay at day 1, 3, 5, 7, 9 after injury. The expression levels of VEGF-C mRNA were quantified with reverse transcription polymerase chain reaction (RT-PCR). The results showed that the lymphatic vessels were found in the injured rat corneas 14 days after the injury. The VEGF-C protein was detectable 3 days after injury, reached the peak 5 days after injury, and gradually decreased. In the control group, no VEGF-C proteins were detected. The VEGF-C mRNA was minimally detected in the normal rat corneas, but it was highly expressed 5 days after the injury. The difference was statistically significant. It was concluded that VEGF-C might be one of the most important relevant factors in corneal lymphangiogenesis after alkali injury.展开更多
To evaluate the efficacy and safety of resection and cryotherapy combined with amniotic membrane transplantation (AMT) for the treatment of vernal keratoconjunctivitis (VKC) with giant papillae (GP). Eight patients (1...To evaluate the efficacy and safety of resection and cryotherapy combined with amniotic membrane transplantation (AMT) for the treatment of vernal keratoconjunctivitis (VKC) with giant papillae (GP). Eight patients (16 eyes involved) with VKC, characterized by GP on the upper tarsal conjunctiva, underwent resection and cryotherapy in combination with AMT. The follow-up lasted for 3-22 months. The results showed that corneal shield ulcers and superficial punctuate keratitis healed during the first week after surgery and did not recur. Fourteen eyes (87.5 %) were symptom-free 1 month after surgery, and no GP, ectropion, trichiasis and other complications were noted, but the blood vessels of upper tarsal conjunctiva could not be clearly seen and a little conjunctival scar was observed. Recurrence of GP was observed in 2 eyes (12.5 %), with the area being less and irritation milder as compared with those before the operation. Among the two eyes, one eye was treated by cyclosporine eyedrops with improvement, but the other eye showed no improvement after the treatment, and underwent a second surgery with a cotton patch soaked in fluorouracil applied onto the su-pratarsal area after resection and cryotherapy. Four months after the treatment the patient presented no symptoms and GP did not recur. It is concluded that the resection and cryotherapy combined with AMT is an effective and safe treatment for VKC with GP.展开更多
文摘目的研究乙酰唑胺对培养的牛角膜内皮细胞液体转运功能的影响。方法观察并记录给予不同浓度乙酰唑胺(0.1μmol/L,1μmol/L,10μmol/L,100μmol/L)后角膜内皮细胞在低渗溶液中的体积改变,并计算其渗透水通透性(osmotic water permeability,Pf)的变化。结果培养的正常牛角膜内皮细胞的Pf值为(0.0446±0.0089)cm/s,在0.1～100μmol/L乙酰唑胺孵育72h后,培养的牛角膜内皮细胞的渗透性水通透性逐渐减少,其Pf值分别为(0.0406±0.0014)cm/s,(0.0364±0.0015)cm/s,(0.0300±0.0022)cm/s,(0.0226±0.0019)cm/s。结论乙酰唑胺呈剂量依赖性地减低角膜内皮渗透性水通透性,抑制角膜内皮细胞液体转运功能。
文摘The expression of VEGF-C and molecular mechanisms of lymphangiogenesis in rat cornea after alkali injury was studied. The rat alkali injured corneal models were made. Under electron microscopy, the lymphatic vessels in the rat injured corneas were examined. The expression of VEGF-C proteins was detected by using immunohistochemical assay at day 1, 3, 5, 7, 9 after injury. The expression levels of VEGF-C mRNA were quantified with reverse transcription polymerase chain reaction (RT-PCR). The results showed that the lymphatic vessels were found in the injured rat corneas 14 days after the injury. The VEGF-C protein was detectable 3 days after injury, reached the peak 5 days after injury, and gradually decreased. In the control group, no VEGF-C proteins were detected. The VEGF-C mRNA was minimally detected in the normal rat corneas, but it was highly expressed 5 days after the injury. The difference was statistically significant. It was concluded that VEGF-C might be one of the most important relevant factors in corneal lymphangiogenesis after alkali injury.
文摘To evaluate the efficacy and safety of resection and cryotherapy combined with amniotic membrane transplantation (AMT) for the treatment of vernal keratoconjunctivitis (VKC) with giant papillae (GP). Eight patients (16 eyes involved) with VKC, characterized by GP on the upper tarsal conjunctiva, underwent resection and cryotherapy in combination with AMT. The follow-up lasted for 3-22 months. The results showed that corneal shield ulcers and superficial punctuate keratitis healed during the first week after surgery and did not recur. Fourteen eyes (87.5 %) were symptom-free 1 month after surgery, and no GP, ectropion, trichiasis and other complications were noted, but the blood vessels of upper tarsal conjunctiva could not be clearly seen and a little conjunctival scar was observed. Recurrence of GP was observed in 2 eyes (12.5 %), with the area being less and irritation milder as compared with those before the operation. Among the two eyes, one eye was treated by cyclosporine eyedrops with improvement, but the other eye showed no improvement after the treatment, and underwent a second surgery with a cotton patch soaked in fluorouracil applied onto the su-pratarsal area after resection and cryotherapy. Four months after the treatment the patient presented no symptoms and GP did not recur. It is concluded that the resection and cryotherapy combined with AMT is an effective and safe treatment for VKC with GP.
