Green fluorescent protein(GFP)gene was inserted into the pseudogene(ψ)region of genome of rabies virus rHep-Flury strain,and a recombinant rabies virus carrying GFP,designated as HEP-GFP,was rescued by reverse geneti...Green fluorescent protein(GFP)gene was inserted into the pseudogene(ψ)region of genome of rabies virus rHep-Flury strain,and a recombinant rabies virus carrying GFP,designated as HEP-GFP,was rescued by reverse genetics system.It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line.The research indicated that the pseudogene(ψ)region in the genome of rHEP-Flury strain,as an independent functional unit in the process of virus assembly,could independently carry and express exogenous genes.展开更多
dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV ...dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV were acquired by RT-PCR amplificationSegment Ⅴ is 2852 nucleotides long and possesses a single open reading frame encoding a putative protein of 881 amino acidsComparison of amino acid sequences shows 97% homology with BmCPV Japanese isolate segments Ⅴ,86% and 36% with LdCPV-1 and LdCPV-14 segments Ⅴ respectivelyPart of sequence of BmCPV segment Ⅴ exhibits high homology with 2Apro motif of picornavirus members,which may suggest that there might exist some affinities evolutionally between both of展开更多
文摘Green fluorescent protein(GFP)gene was inserted into the pseudogene(ψ)region of genome of rabies virus rHep-Flury strain,and a recombinant rabies virus carrying GFP,designated as HEP-GFP,was rescued by reverse genetics system.It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line.The research indicated that the pseudogene(ψ)region in the genome of rHEP-Flury strain,as an independent functional unit in the process of virus assembly,could independently carry and express exogenous genes.
文摘dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV were acquired by RT-PCR amplificationSegment Ⅴ is 2852 nucleotides long and possesses a single open reading frame encoding a putative protein of 881 amino acidsComparison of amino acid sequences shows 97% homology with BmCPV Japanese isolate segments Ⅴ,86% and 36% with LdCPV-1 and LdCPV-14 segments Ⅴ respectivelyPart of sequence of BmCPV segment Ⅴ exhibits high homology with 2Apro motif of picornavirus members,which may suggest that there might exist some affinities evolutionally between both of
