A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is built ...A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time.The shifts of resonant wavelength at different reaction time stages are obtained by SPR,corresponding well with the changes of the fluorescence intensity collected by using LSCM.The instrument shows the merits of the combination and complementation of the SPR and LSCM,with such advantages as quantificational analysis,high spatial resolution and real time monitor,which are of great importance for practical applications in biosensor and life science.展开更多
基金supported by the Instrument Developing Project of the Chinese Academy of Sciences (Grant No.YZ200740)the National Natural Science Foundation of China (Grant Nos.60978034 and 10974019)the National High Technology Research and Development Program of China (Grant No.2009AA03Z318)
文摘A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time.The shifts of resonant wavelength at different reaction time stages are obtained by SPR,corresponding well with the changes of the fluorescence intensity collected by using LSCM.The instrument shows the merits of the combination and complementation of the SPR and LSCM,with such advantages as quantificational analysis,high spatial resolution and real time monitor,which are of great importance for practical applications in biosensor and life science.
