为实现小反刍兽疫病毒(Peste des petits ruminants virus,PPRV)的快速、准确、定量检测,以国内PPRV Clone 9疫苗株N基因为靶位点设计特异性引物及探针,建立了液滴式数字PCR(Droplet digital PCR,ddPCR)方法,并对其特异性、敏感性及重...为实现小反刍兽疫病毒(Peste des petits ruminants virus,PPRV)的快速、准确、定量检测,以国内PPRV Clone 9疫苗株N基因为靶位点设计特异性引物及探针,建立了液滴式数字PCR(Droplet digital PCR,ddPCR)方法,并对其特异性、敏感性及重复性进行评价。将所建立的ddPCR方法与实时荧光定量PCR(Fluorescence quantitative PCR,qPCR)方法的灵敏度进行比较分析并应用于PPRV(Clone 9株)核糖核酸标准物质的定量。结果显示,所建立的ddPCR方法特异性好,仅PPRV检测结果为阳性,其他羊源病毒类生物制品及毒种检测结果均为阴性;敏感性高,基因拷贝数检出限度为4.33拷贝/μL,比qPCR方法的灵敏度(57.3拷贝/μL)高10倍;重复性好,重复性试验的变异系数为1.8%;定量准确,具有一定资质的9家单位各组测量数据组间变异系数小于5%。试验建立的ddPCR方法能够有效地对PPRV核酸进行快速检测,为PPRV的诊断及绝对定量提供了有效方法。展开更多
卵巢局部环境与多种因素相关,其局部微环境异常与多囊卵巢综合征(Polycystic ovary syndrome, PCOS)有着密切联系。PCOS临床常表现为慢性低度炎症状态、月经后期、卵巢局部菌群异常、胰岛素抵抗以及卵泡发育异常、排卵功能障碍,这些与P...卵巢局部环境与多种因素相关,其局部微环境异常与多囊卵巢综合征(Polycystic ovary syndrome, PCOS)有着密切联系。PCOS临床常表现为慢性低度炎症状态、月经后期、卵巢局部菌群异常、胰岛素抵抗以及卵泡发育异常、排卵功能障碍,这些与PCOS卵巢局部环境异常关系密切。本文主要探讨卵巢局部的微环境异常对PCOS的影响及其作用机制,从卵巢角度对PCOS的影响进行总结,分析PCOS发生与卵巢的相关性,以期为PCOS相关研究提供参考。The local environment of ovary is related to many factors, and the abnormal local microenvironment is closely related to Polycystic ovary syndrome, PCOS). Clinical manifestations of PCOS are chronic low-grade inflammation, late menstruation, abnormal local flora of ovary, insulin resistance, abnormal follicular development and ovulation dysfunction, which are closely related to abnormal local ovarian environment of PCOS. This paper mainly discusses the influence of local microenvironment abnormality on PCOS and its mechanism, summarizes the influence on PCOS from the perspective of ovary, and analyzes the correlation between PCOS and ovary, so as to provide reference for PCOS related research.展开更多
文摘卵巢局部环境与多种因素相关,其局部微环境异常与多囊卵巢综合征(Polycystic ovary syndrome, PCOS)有着密切联系。PCOS临床常表现为慢性低度炎症状态、月经后期、卵巢局部菌群异常、胰岛素抵抗以及卵泡发育异常、排卵功能障碍,这些与PCOS卵巢局部环境异常关系密切。本文主要探讨卵巢局部的微环境异常对PCOS的影响及其作用机制,从卵巢角度对PCOS的影响进行总结,分析PCOS发生与卵巢的相关性,以期为PCOS相关研究提供参考。The local environment of ovary is related to many factors, and the abnormal local microenvironment is closely related to Polycystic ovary syndrome, PCOS). Clinical manifestations of PCOS are chronic low-grade inflammation, late menstruation, abnormal local flora of ovary, insulin resistance, abnormal follicular development and ovulation dysfunction, which are closely related to abnormal local ovarian environment of PCOS. This paper mainly discusses the influence of local microenvironment abnormality on PCOS and its mechanism, summarizes the influence on PCOS from the perspective of ovary, and analyzes the correlation between PCOS and ovary, so as to provide reference for PCOS related research.
