Objective: To evaluate penetration capacity of human sperm preserved in electrolyte-free (EF) solution at 4 ℃.Methods: The motility, acrosomal status penetration rate and fertility index of human sperm were assessed ...Objective: To evaluate penetration capacity of human sperm preserved in electrolyte-free (EF) solution at 4 ℃.Methods: The motility, acrosomal status penetration rate and fertility index of human sperm were assessed before and after cold-preservation in EF solution, respectively.Results: The motility of human sperm cold-preserved in EF solution for 1 week was significantly higher than that of human sperm cold-preserved in modified human tubal fluid (mHTF) (43.4%±7.9% vs 9.5%±2.5%, P<0.01 ).Although acrosomal status of human sperm cold-preserved in the EF solution before reinitiation was not different from those of the fresh sperm (capacitated sperm: 7.6%±1.8% vs 6.4±1.8%; acrosome-reacted sperm: 3.0%±1.7% vs 2.4±1.1%, P>0.05), the percentage of capacitated and acrosome-reacted sperm in the EF solution significantly increased after reinitiation (capacitated sperm: 16.0%±2.3% vs 7.6±1.8%, acrosome-reacted sperm: 9.4%±2.1% vs 3.0%±1.7%, P<0.01).The penetration rate and fertility index of cool-preserved human sperm in the EF solution were comparable with those of fresh sperm (48.1% vs 50.9%; 1.38±0.16 vs 1.29±0.13, respectively, P>0.05).Conclusion: Cold-preservation did not induce capacitation and acrosome reaction of human sperm in the EF solution, but human sperm cold-preserved in the EF solution for 1 week possesses as much penetration capacity as fresh sperm.展开更多
Objective: To assess the developing potentiality of mouse morula produced in vitro or in the after vitrification and to evaluate the effect of one-step and two-step vitrification methods. Method: Mouse morula produced...Objective: To assess the developing potentiality of mouse morula produced in vitro or in the after vitrification and to evaluate the effect of one-step and two-step vitrification methods. Method: Mouse morula produced in for and in the were vitrified in the solution containing ethylene glycol, Ficoll and sucrose (EFS solution) with one-step and two-step methods. The developing potential and status of the pellucid zona in vitified mouse morula were assessed. Results: The percentages of morula developed into blastocyst stage were 81. 8% and 82.4%, 97. 3% and 98.4%, respectively, after one-step and two-step exposure of in vitro morula or in vivo morula to EFS solution alone, which did not show significant difference compared to their controls (P > 0. 05). The percentage of in vitro morula developed into blastocyst vitrified by onestep method was significantly lower than that by two-step method and coned (P < 0.05, 70.6% vs 81 .3%; 70.6% vs 83 .6%, respectively). However, there was no significant difference between blastocyst rates of in vivo morula vitrified by one-step and two-step methods (P>0.05, 93. 1% us 95.7%). No rupture of pellucid zona was observed in all thawed morula after one-step and two-step vitrification, irrespective of in vitro morula or in vivo morula. Conclusion: Morula produced in vitro and in vivo after vitrification may maintain high survival rate and developing potential. Two-step vitrification method with EFS solution is suitable for in vitro morula, which can improve the developing potential of in vitro morula. Onestep and two-step vitrification method have no detrimennd effect on the developing potential of in vivo morula.展开更多
文摘Objective: To evaluate penetration capacity of human sperm preserved in electrolyte-free (EF) solution at 4 ℃.Methods: The motility, acrosomal status penetration rate and fertility index of human sperm were assessed before and after cold-preservation in EF solution, respectively.Results: The motility of human sperm cold-preserved in EF solution for 1 week was significantly higher than that of human sperm cold-preserved in modified human tubal fluid (mHTF) (43.4%±7.9% vs 9.5%±2.5%, P<0.01 ).Although acrosomal status of human sperm cold-preserved in the EF solution before reinitiation was not different from those of the fresh sperm (capacitated sperm: 7.6%±1.8% vs 6.4±1.8%; acrosome-reacted sperm: 3.0%±1.7% vs 2.4±1.1%, P>0.05), the percentage of capacitated and acrosome-reacted sperm in the EF solution significantly increased after reinitiation (capacitated sperm: 16.0%±2.3% vs 7.6±1.8%, acrosome-reacted sperm: 9.4%±2.1% vs 3.0%±1.7%, P<0.01).The penetration rate and fertility index of cool-preserved human sperm in the EF solution were comparable with those of fresh sperm (48.1% vs 50.9%; 1.38±0.16 vs 1.29±0.13, respectively, P>0.05).Conclusion: Cold-preservation did not induce capacitation and acrosome reaction of human sperm in the EF solution, but human sperm cold-preserved in the EF solution for 1 week possesses as much penetration capacity as fresh sperm.
文摘Objective: To assess the developing potentiality of mouse morula produced in vitro or in the after vitrification and to evaluate the effect of one-step and two-step vitrification methods. Method: Mouse morula produced in for and in the were vitrified in the solution containing ethylene glycol, Ficoll and sucrose (EFS solution) with one-step and two-step methods. The developing potential and status of the pellucid zona in vitified mouse morula were assessed. Results: The percentages of morula developed into blastocyst stage were 81. 8% and 82.4%, 97. 3% and 98.4%, respectively, after one-step and two-step exposure of in vitro morula or in vivo morula to EFS solution alone, which did not show significant difference compared to their controls (P > 0. 05). The percentage of in vitro morula developed into blastocyst vitrified by onestep method was significantly lower than that by two-step method and coned (P < 0.05, 70.6% vs 81 .3%; 70.6% vs 83 .6%, respectively). However, there was no significant difference between blastocyst rates of in vivo morula vitrified by one-step and two-step methods (P>0.05, 93. 1% us 95.7%). No rupture of pellucid zona was observed in all thawed morula after one-step and two-step vitrification, irrespective of in vitro morula or in vivo morula. Conclusion: Morula produced in vitro and in vivo after vitrification may maintain high survival rate and developing potential. Two-step vitrification method with EFS solution is suitable for in vitro morula, which can improve the developing potential of in vitro morula. Onestep and two-step vitrification method have no detrimennd effect on the developing potential of in vivo morula.
