目的:探讨环磷酸腺苷反应元件结合蛋白(cAMP response element binding protein,p-CREB)在切割痛敏形成中的作用。方法:异氟烷麻醉大鼠后切割后足。免疫组织化学染色,荧光双标对p-CREB进行定位。腹腔注射吗啡和加巴喷丁,进行行为学测定...目的:探讨环磷酸腺苷反应元件结合蛋白(cAMP response element binding protein,p-CREB)在切割痛敏形成中的作用。方法:异氟烷麻醉大鼠后切割后足。免疫组织化学染色,荧光双标对p-CREB进行定位。腹腔注射吗啡和加巴喷丁,进行行为学测定和免疫组织化学染色。结果:大鼠后足切割痛可以使同侧腰段脊髓背角内p-CREB增加,切割后0.5～3 h内比较明显(P<0.05)。p-CREB增加主要定位于背角神经元。腹腔注射吗啡产生镇痛作用的同时,与未切割组相比,p-CREB没有出现增加(P>0.05)。加巴喷丁产生部分镇痛作用时,与未切割组相比,p-CREB增加(P<0.05)。结论:切割疼痛所致的同侧脊髓背角内p-CREB增加与切割疼痛和痛觉过敏密切相关。吗啡对切割疼痛的镇痛作用机制与抑制脊髓背角内CREB的磷酸化增加有关。加巴喷丁对切割疼痛的镇痛作用机制与抑制脊髓背角内CREB磷酸化关系不密切。展开更多
Fiber gas lasers based on gas-filled hollow-core fibers(HCFs)perfectly combine the advantages of fiber lasers and gas lasers and have obtained fast development in the past years.However,stable and efficient coupling o...Fiber gas lasers based on gas-filled hollow-core fibers(HCFs)perfectly combine the advantages of fiber lasers and gas lasers and have obtained fast development in the past years.However,stable and efficient coupling of high-power pump lasers into the HCFs is one of the key problems to be solved.In this paper,we study the coupling of high-power continuous wave fiber lasers into anti-resonant HCFs through an end-cap.By optimizing the splicing parameters,a maximum laser power of 1167 W was injected into the 1-m-long HCFs,and 1021W was obtained at the output end,giving a total transmission efficiency of〜87.5%.A more than 1 h test showed the stability of such a coupling method.Meanwhile,the laser beam quality was well maintained.This work opens new opportunities for stable and highly efficient coupling of high-power lasers into HCFs,which is significant for its applications in many other fields besides high-power fiber gas lasers,such as high-power laser delivering.展开更多
基金This work was supported by the Outstanding Youth Science Fund of Hunan Provincial Natural Science Foundation(No.2019JJ20023)National Natural Science Foundation of China(NSFC)(Nos.11974427 and 12004431)+2 种基金State Key Laboratory of Pulsed Power Laser(Nos.SKL2020ZR05 and SKL2021ZR01)Science and Technology Innovation Program of Hunan Province(No.2021RC4027)Postgraduate Scientific Research Innovation Project of Hunan Province(No.CX20200017).
文摘Fiber gas lasers based on gas-filled hollow-core fibers(HCFs)perfectly combine the advantages of fiber lasers and gas lasers and have obtained fast development in the past years.However,stable and efficient coupling of high-power pump lasers into the HCFs is one of the key problems to be solved.In this paper,we study the coupling of high-power continuous wave fiber lasers into anti-resonant HCFs through an end-cap.By optimizing the splicing parameters,a maximum laser power of 1167 W was injected into the 1-m-long HCFs,and 1021W was obtained at the output end,giving a total transmission efficiency of〜87.5%.A more than 1 h test showed the stability of such a coupling method.Meanwhile,the laser beam quality was well maintained.This work opens new opportunities for stable and highly efficient coupling of high-power lasers into HCFs,which is significant for its applications in many other fields besides high-power fiber gas lasers,such as high-power laser delivering.
