In this paper, we report an integrated microfluidic chip for drug-metabolism. The chip contains three layers: the top PDMS cover, the middle quartz slide with microchannels and microwells, the bottom PDMS layer for ce...In this paper, we report an integrated microfluidic chip for drug-metabolism. The chip contains three layers: the top PDMS cover, the middle quartz slide with microchannels and microwells, the bottom PDMS layer for cell culture. Microsomes isolated from human liver were encapsulated in microwells by sol-gel method.Using this design, the determination of the metabolites and drug cytotoxicity assessment can be carried out within a single microfluidic device, providing an alternative technique for the high-throughput parallel drug screening and drug interactions in the metabolic pathways.展开更多
文摘In this paper, we report an integrated microfluidic chip for drug-metabolism. The chip contains three layers: the top PDMS cover, the middle quartz slide with microchannels and microwells, the bottom PDMS layer for cell culture. Microsomes isolated from human liver were encapsulated in microwells by sol-gel method.Using this design, the determination of the metabolites and drug cytotoxicity assessment can be carried out within a single microfluidic device, providing an alternative technique for the high-throughput parallel drug screening and drug interactions in the metabolic pathways.
