本文报导了武汉东湖水体中病毒和指示细菌存在的水平。研究结果表明东湖水体中病原体污染严重。所检测的56个样品中,有24个样品检出病毒,检出率为42.8%.含量范围为0～167.5Pfu/L,平均为4.97Pfu/L.已鉴定出的病毒有脊髓灰质炎病毒,柯萨奇...本文报导了武汉东湖水体中病毒和指示细菌存在的水平。研究结果表明东湖水体中病原体污染严重。所检测的56个样品中,有24个样品检出病毒,检出率为42.8%.含量范围为0～167.5Pfu/L,平均为4.97Pfu/L.已鉴定出的病毒有脊髓灰质炎病毒,柯萨奇 B 组病毒和腺病毒。由于东湖水体的病毒和细菌严重污染,它已影响了作为饮用水水源和旅游用水的功能。展开更多
According to the sequence of gD gene of PRV Rice strain, the primers of 22bp were designed.Using PRV genomic DNA of Hubei and Shuangcheng virus strains which infected BHK 21 cell separately as template, the gD...According to the sequence of gD gene of PRV Rice strain, the primers of 22bp were designed.Using PRV genomic DNA of Hubei and Shuangcheng virus strains which infected BHK 21 cell separately as template, the gD gene of PRV was amplified sucessfully by PCR and cloned into pGEM T vector. Restriction enzyme analysis showed that the cloned gD gene at SmaⅠ,SalⅠ,KpnⅠ,PvuⅡ sites was the same as that of PRV Rice strain. The gD gene consisted of 1,263 nucleotides including an open reading frame spanning 1,197 nucleotieds which could encode a protein of 398 amino acids. The ORF didn′t include an amino acid sequence directing N linked glycosylation (NXT or NXS). Comparison of our complete Hubei strain gD gene sequence with the Rice strain gD gene sequence showed that the nucleotide and deduced amino acid homology were about 97% and there was an 12 basepair deletion in 835 846 nucleotide sites that coded Arg Pro Arg Pro. A region of the amino acid sequence and the positions of the cysteine residues of PRV HB gD were homologous to HSV I glycoprotein D. This work laid foundation for PRV gene immunization and studying PRV sub unit vaccine.展开更多
文摘本文报导了武汉东湖水体中病毒和指示细菌存在的水平。研究结果表明东湖水体中病原体污染严重。所检测的56个样品中,有24个样品检出病毒,检出率为42.8%.含量范围为0～167.5Pfu/L,平均为4.97Pfu/L.已鉴定出的病毒有脊髓灰质炎病毒,柯萨奇 B 组病毒和腺病毒。由于东湖水体的病毒和细菌严重污染,它已影响了作为饮用水水源和旅游用水的功能。
文摘According to the sequence of gD gene of PRV Rice strain, the primers of 22bp were designed.Using PRV genomic DNA of Hubei and Shuangcheng virus strains which infected BHK 21 cell separately as template, the gD gene of PRV was amplified sucessfully by PCR and cloned into pGEM T vector. Restriction enzyme analysis showed that the cloned gD gene at SmaⅠ,SalⅠ,KpnⅠ,PvuⅡ sites was the same as that of PRV Rice strain. The gD gene consisted of 1,263 nucleotides including an open reading frame spanning 1,197 nucleotieds which could encode a protein of 398 amino acids. The ORF didn′t include an amino acid sequence directing N linked glycosylation (NXT or NXS). Comparison of our complete Hubei strain gD gene sequence with the Rice strain gD gene sequence showed that the nucleotide and deduced amino acid homology were about 97% and there was an 12 basepair deletion in 835 846 nucleotide sites that coded Arg Pro Arg Pro. A region of the amino acid sequence and the positions of the cysteine residues of PRV HB gD were homologous to HSV I glycoprotein D. This work laid foundation for PRV gene immunization and studying PRV sub unit vaccine.
