目的:初步探讨凋亡“核心蛋白酶”———Caspase-3在槟榔碱(Arecoline)诱导血管内皮细胞(EndothelialCells,EC)凋亡中的作用。方法:体外培养人脐静脉内皮细胞(Hum an Umb ilicalVe in Endothelial Cells,HUVECs),将EC与槟榔碱共同培养,...目的:初步探讨凋亡“核心蛋白酶”———Caspase-3在槟榔碱(Arecoline)诱导血管内皮细胞(EndothelialCells,EC)凋亡中的作用。方法:体外培养人脐静脉内皮细胞(Hum an Umb ilicalVe in Endothelial Cells,HUVECs),将EC与槟榔碱共同培养,应用荧光显微镜观察EC凋亡情况,计算凋亡百分率并用比色法检测Caspase-3的活性。结果:槟榔碱诱导血管内皮细胞组在荧光显微镜下可见到明显的细胞凋亡改变,Caspase-3活性较正常对照组明显增高(P<0.05),且在12 h时最高。结论:槟榔碱可诱导血管内皮细胞凋亡,Caspase-3可能参与了这一细胞凋亡过程的调控。展开更多
Objectie:To investigate the relationship between psychological factors and burning mouth syndrome (BMS).Methods: Eighty-seven consecutive patients with BMS and a randomly selected control group( n =82) were evaluated ...Objectie:To investigate the relationship between psychological factors and burning mouth syndrome (BMS).Methods: Eighty-seven consecutive patients with BMS and a randomly selected control group( n =82) were evaluated by a self-designed questionnaire,the Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS).Results: BMS group reported negative life events more frequently than the control group.Patients with BMS significantly exhibited more symptoms of somatization,and both the scores of anxiety,depression in the BMS patients were higher than those of the control group( P <0.05).Conclusion:The results of this study indicated that BMS may be of psychological origin.展开更多
文摘目的:初步探讨凋亡“核心蛋白酶”———Caspase-3在槟榔碱(Arecoline)诱导血管内皮细胞(EndothelialCells,EC)凋亡中的作用。方法:体外培养人脐静脉内皮细胞(Hum an Umb ilicalVe in Endothelial Cells,HUVECs),将EC与槟榔碱共同培养,应用荧光显微镜观察EC凋亡情况,计算凋亡百分率并用比色法检测Caspase-3的活性。结果:槟榔碱诱导血管内皮细胞组在荧光显微镜下可见到明显的细胞凋亡改变,Caspase-3活性较正常对照组明显增高(P<0.05),且在12 h时最高。结论:槟榔碱可诱导血管内皮细胞凋亡,Caspase-3可能参与了这一细胞凋亡过程的调控。
文摘Objectie:To investigate the relationship between psychological factors and burning mouth syndrome (BMS).Methods: Eighty-seven consecutive patients with BMS and a randomly selected control group( n =82) were evaluated by a self-designed questionnaire,the Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS).Results: BMS group reported negative life events more frequently than the control group.Patients with BMS significantly exhibited more symptoms of somatization,and both the scores of anxiety,depression in the BMS patients were higher than those of the control group( P <0.05).Conclusion:The results of this study indicated that BMS may be of psychological origin.
