[Objective] The paper aimed to study the control effects of live spore preparations of Trichoderma viride strains against vegetable grey mold in greenhouse. [Method] Trichoderma viride strains NW-411 live spore prepar...[Objective] The paper aimed to study the control effects of live spore preparations of Trichoderma viride strains against vegetable grey mold in greenhouse. [Method] Trichoderma viride strains NW-411 live spore preparations were prepared by solid-state fermentation,106-107 spore/g diluent was made to conduct field control experiment,traits change of cucumber and tomato plants inoculated grey mold were investigated,control effect was calculated. [Result] Cucumber and tomato plants without dilution treatment of T. viride spores could be infected with different changes in trait. T. viride spore preparations had a significant preventive effect on greenhouse cucumber and tomato gray mold,the optimal concentration of spores was in the range of 2.3×10^6-2.3×10^7 spore/g. The incidence of cucumber and tomato plants were reduced to 4.2% and 3.1%,the incidence rate decreased 29.8% and 39.1% compared with plants without treatment,biological control effect was over 87%,and the plant growth can be enhanced obviously. [Conclusion] Live spores preparation of T. viride not only had a significant effect on grey mold,but also significantly enhanced the plants growth in greenhouse,which is a safety and environmental protection biological agent,and worthy to be widely spread in large-scale green vegetable production.展开更多
Chalcone synthase A is a key enzyme in the anthocyanin biosynthesis pathway. Expression of chsA gene in transgenic Petunia hybrida resulted in flower color alterations and co-suppression of transgenes and endogenous g...Chalcone synthase A is a key enzyme in the anthocyanin biosynthesis pathway. Expression of chsA gene in transgenic Petunia hybrida resulted in flower color alterations and co-suppression of transgenes and endogenous genes. We fused the β-glucuronidase (uidA) gene to the C-terminal of chsA gene, and transferred the fusion gene into Petunia hybrida via Agrobacterium tumefaciens. GUS histochemical staining analysis showed that co-suppression occurred specifically during the development of flowers and co-suppression required the mutual interaction of endogenous genes and transgenes. RNA in situ hybridization analysis suggested that co-suppression occurred in the entire plant, and RNA degradation occurred in the cytoplasm.展开更多
基金Supported by Scientific and Technological Project in Shaanxi Province(2001K02-G7)~~
文摘[Objective] The paper aimed to study the control effects of live spore preparations of Trichoderma viride strains against vegetable grey mold in greenhouse. [Method] Trichoderma viride strains NW-411 live spore preparations were prepared by solid-state fermentation,106-107 spore/g diluent was made to conduct field control experiment,traits change of cucumber and tomato plants inoculated grey mold were investigated,control effect was calculated. [Result] Cucumber and tomato plants without dilution treatment of T. viride spores could be infected with different changes in trait. T. viride spore preparations had a significant preventive effect on greenhouse cucumber and tomato gray mold,the optimal concentration of spores was in the range of 2.3×10^6-2.3×10^7 spore/g. The incidence of cucumber and tomato plants were reduced to 4.2% and 3.1%,the incidence rate decreased 29.8% and 39.1% compared with plants without treatment,biological control effect was over 87%,and the plant growth can be enhanced obviously. [Conclusion] Live spores preparation of T. viride not only had a significant effect on grey mold,but also significantly enhanced the plants growth in greenhouse,which is a safety and environmental protection biological agent,and worthy to be widely spread in large-scale green vegetable production.
基金the National Natural Science Foundation of China (Grant No. 39670415), Yunnan Province-Peking University joint project (B9810K) and Shenzhen Science and Technology Bureau.
文摘Chalcone synthase A is a key enzyme in the anthocyanin biosynthesis pathway. Expression of chsA gene in transgenic Petunia hybrida resulted in flower color alterations and co-suppression of transgenes and endogenous genes. We fused the β-glucuronidase (uidA) gene to the C-terminal of chsA gene, and transferred the fusion gene into Petunia hybrida via Agrobacterium tumefaciens. GUS histochemical staining analysis showed that co-suppression occurred specifically during the development of flowers and co-suppression required the mutual interaction of endogenous genes and transgenes. RNA in situ hybridization analysis suggested that co-suppression occurred in the entire plant, and RNA degradation occurred in the cytoplasm.
