目的探讨供体NKT细胞静脉输注诱导移植耐受的作用及其机制。方法C57BL/6和BALB/c小鼠分别为供体和受体,建立皮肤移植模型。对照组:术后注射生理盐水;NKT组:术前静脉注射供体鼠NKT细胞(5×106个/只),术后腹腔注射生理盐水;CsA组:术...目的探讨供体NKT细胞静脉输注诱导移植耐受的作用及其机制。方法C57BL/6和BALB/c小鼠分别为供体和受体,建立皮肤移植模型。对照组:术后注射生理盐水;NKT组:术前静脉注射供体鼠NKT细胞(5×106个/只),术后腹腔注射生理盐水;CsA组:术后腹腔注射环孢素(CsA)(4mg/kg);NKT+CsA组:手术前1日静脉注射供体鼠NKT细胞(5×106个/只),术后腹腔注射CsA(4mg/kg)。术后逐日观察移植皮肤存活情况及小鼠一般状况;术后14天对植皮BALB/c小鼠做混合淋巴细胞反应(mixed lymphocyte reaction,MLR)、迟发型超敏反应(delayed type hypersensitivity,DTH)等确定耐受的状态;并通过过继转移实验及脾细胞中细胞因子mRNA表达情况,进一步探讨耐受形成的机制。结果采用此诱导方案,移植皮片存活时间明显延长,耐受可被过继转移,Th2型细胞因子IL-10、IL-4在耐受小鼠中明显升高。结论供体NKT细胞静脉输注能诱导异基因小鼠皮肤移植耐受,抑制细胞、Th1/Th2偏移在耐受中起了一定作用。展开更多
Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control...Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control,sham operation, model and EA groups, and each of them were further divided into IR 12 hr, IR 24 hr and IR 48 hr subgroups, with 10 cases being in each subgroup. Cerebral ischemia was produced by occlusion of the middle cerebral artery for 0.5 hr. Shuigou (GV 26) and Baihui (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus. Serum IL 1β and tumor necrosis factor (TNF) α contents were assayed by using radioimmunoassay. Results: Results showed that serum IL 1β and TNF α contents of model group were significantly higher than those of normal control and sham operation groups at IR 12 hr, 24 hr and 48 hr (P<0.05), meaning that following IR, the production and release of these two cytokines in the blood increased obviously; comparison between EA and model groups displayed that serum IL 1β and TNF α levels of EA group were strikingly lower than those of model group (P<0.01), suggesting that EA stimulation can suppress IR induced increase of production and release of these two cytokines. Conclusion: EA can suppress cerebral IR induced increase of serum IL 1β and TNF α, which may contribute to the effect of acupuncture in resisting cerebral IR injury in the treatment of stroke.展开更多
文摘目的探讨供体NKT细胞静脉输注诱导移植耐受的作用及其机制。方法C57BL/6和BALB/c小鼠分别为供体和受体,建立皮肤移植模型。对照组:术后注射生理盐水;NKT组:术前静脉注射供体鼠NKT细胞(5×106个/只),术后腹腔注射生理盐水;CsA组:术后腹腔注射环孢素(CsA)(4mg/kg);NKT+CsA组:手术前1日静脉注射供体鼠NKT细胞(5×106个/只),术后腹腔注射CsA(4mg/kg)。术后逐日观察移植皮肤存活情况及小鼠一般状况;术后14天对植皮BALB/c小鼠做混合淋巴细胞反应(mixed lymphocyte reaction,MLR)、迟发型超敏反应(delayed type hypersensitivity,DTH)等确定耐受的状态;并通过过继转移实验及脾细胞中细胞因子mRNA表达情况,进一步探讨耐受形成的机制。结果采用此诱导方案,移植皮片存活时间明显延长,耐受可被过继转移,Th2型细胞因子IL-10、IL-4在耐受小鼠中明显升高。结论供体NKT细胞静脉输注能诱导异基因小鼠皮肤移植耐受,抑制细胞、Th1/Th2偏移在耐受中起了一定作用。
文摘Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control,sham operation, model and EA groups, and each of them were further divided into IR 12 hr, IR 24 hr and IR 48 hr subgroups, with 10 cases being in each subgroup. Cerebral ischemia was produced by occlusion of the middle cerebral artery for 0.5 hr. Shuigou (GV 26) and Baihui (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus. Serum IL 1β and tumor necrosis factor (TNF) α contents were assayed by using radioimmunoassay. Results: Results showed that serum IL 1β and TNF α contents of model group were significantly higher than those of normal control and sham operation groups at IR 12 hr, 24 hr and 48 hr (P<0.05), meaning that following IR, the production and release of these two cytokines in the blood increased obviously; comparison between EA and model groups displayed that serum IL 1β and TNF α levels of EA group were strikingly lower than those of model group (P<0.01), suggesting that EA stimulation can suppress IR induced increase of production and release of these two cytokines. Conclusion: EA can suppress cerebral IR induced increase of serum IL 1β and TNF α, which may contribute to the effect of acupuncture in resisting cerebral IR injury in the treatment of stroke.
