The full coding region of brain derived neurotrophic factor (BDNF) gene from Tylototriton taliangensis genome was amplified by PCR with the primers designed according to the sequence of human BDNF gene. Sequence analy...The full coding region of brain derived neurotrophic factor (BDNF) gene from Tylototriton taliangensis genome was amplified by PCR with the primers designed according to the sequence of human BDNF gene. Sequence analysis shows that the coding region has 744 bp in length and is highly homologous with that of mammals and aves. The homology of deduced BDNF protein sequence is about 90% with that of human. It is the first time to amplify and analyze BDNF gene from the newt genome, which will provide useful information for protecting and studying the severely endangered species. Fig1,Tab1,Ref展开更多
文摘The full coding region of brain derived neurotrophic factor (BDNF) gene from Tylototriton taliangensis genome was amplified by PCR with the primers designed according to the sequence of human BDNF gene. Sequence analysis shows that the coding region has 744 bp in length and is highly homologous with that of mammals and aves. The homology of deduced BDNF protein sequence is about 90% with that of human. It is the first time to amplify and analyze BDNF gene from the newt genome, which will provide useful information for protecting and studying the severely endangered species. Fig1,Tab1,Ref
