During conjugation of Paramecium caudatum, nuclear events occur in a scheduled program. Morphological studies on nuclear behavior during conjugation of P. caudatum have been performed since the end of the 19th century...During conjugation of Paramecium caudatum, nuclear events occur in a scheduled program. Morphological studies on nuclear behavior during conjugation of P. caudatum have been performed since the end of the 19th century. Here we report on new details concerning the conjugation of P. caudatum through the staining of conjugating ceils with protargol, carbol fuchsin solution, Hoechst 33342 and immunofluorescence labeling with monoclonal antibody of anti-a tubulin. 1） The crescent nucleus is a characteristic of the meiotic prophase of P. caudatum, has an unstained area. We stained this area with protargol, which was separated from the chromatin area and was not detected by the other stainings. 2） In regards to the four meiotic products, it has long been considered that only one product enters the paroral cone region （PC） and survives after meiosis. However, our protargol and immunofluorescence labeling results indicated that PC entrance of the meiotic product happened before the completion of meiosis instead of after. 3） In our previous study, protargol staining indicated the presence of a swollen structure around the central part of the ＂U＂ and ＂V＂ shaped spindles connecting the two types of prospective pronuclei. However, immunofluorescence labeling with anti-a tubulin antibodies gave a different image from protargol. All these observations form the basis for further studies of their molecular mechanisms.展开更多
After the third prezygotic division during conjugation of Paramecium caudatum,migratory and stationary pronuclei are produced.The migratory pronuclei remain in the paroral region tightly against the conjugating bounda...After the third prezygotic division during conjugation of Paramecium caudatum,migratory and stationary pronuclei are produced.The migratory pronuclei remain in the paroral region tightly against the conjugating boundaries;while the stationary pronuclei are located beside the migratory pronuclei.To date,however,it is not clear what causes this close side-by-side localization between migratory and stationary pronuclei.In the current study,immunofluorescence staining with monoclonal antibody of anti-α tubulin indicated that ＂U＂ or ＂V＂ shaped spindles connected the migratory and stationary pronuclei during the third prezygotic division.This observation accounts for the close localization between these two types of pronuclei.展开更多
目的:检测MMP-2、BMP-2基因在口腔颌面部骨化性纤维瘤(Oral and Maxillofacial ossifying fibroma,OMOF)中的表达情况,分析其与肿瘤病理分型、临床分级之间的关系,并探讨其作为OMOF基因治疗靶基因的可能性。方法:采用免疫组织化学EnVis...目的:检测MMP-2、BMP-2基因在口腔颌面部骨化性纤维瘤(Oral and Maxillofacial ossifying fibroma,OMOF)中的表达情况,分析其与肿瘤病理分型、临床分级之间的关系,并探讨其作为OMOF基因治疗靶基因的可能性。方法:采用免疫组织化学EnVision法检测27例OMOF标本及8例颌骨非肿瘤组织中MMP-2、BMP-2基因的表达,SPSS统计软件分析所得数据。结果:27例OMOF标本中9例BMP-2阳性表达(33.3%),18例MMP-2阳性表达(66.7%),均定位于细胞浆与细胞核;而8例非肿瘤骨组织无一例表达;MMP-2蛋白的表达与肿瘤大小、临床分型、患者年龄与性别均无关,BMP-2蛋白的表达与肿瘤大小及年龄、临床病理分型有关(P<0.05),但与患者性别无关。结论:MMP-2蛋白比BMP-2蛋白在OMOF组织中的表达更高,因此将其作为研究OMOF免疫治疗靶点具有一定可行性。展开更多
文摘During conjugation of Paramecium caudatum, nuclear events occur in a scheduled program. Morphological studies on nuclear behavior during conjugation of P. caudatum have been performed since the end of the 19th century. Here we report on new details concerning the conjugation of P. caudatum through the staining of conjugating ceils with protargol, carbol fuchsin solution, Hoechst 33342 and immunofluorescence labeling with monoclonal antibody of anti-a tubulin. 1） The crescent nucleus is a characteristic of the meiotic prophase of P. caudatum, has an unstained area. We stained this area with protargol, which was separated from the chromatin area and was not detected by the other stainings. 2） In regards to the four meiotic products, it has long been considered that only one product enters the paroral cone region （PC） and survives after meiosis. However, our protargol and immunofluorescence labeling results indicated that PC entrance of the meiotic product happened before the completion of meiosis instead of after. 3） In our previous study, protargol staining indicated the presence of a swollen structure around the central part of the ＂U＂ and ＂V＂ shaped spindles connecting the two types of prospective pronuclei. However, immunofluorescence labeling with anti-a tubulin antibodies gave a different image from protargol. All these observations form the basis for further studies of their molecular mechanisms.
文摘After the third prezygotic division during conjugation of Paramecium caudatum,migratory and stationary pronuclei are produced.The migratory pronuclei remain in the paroral region tightly against the conjugating boundaries;while the stationary pronuclei are located beside the migratory pronuclei.To date,however,it is not clear what causes this close side-by-side localization between migratory and stationary pronuclei.In the current study,immunofluorescence staining with monoclonal antibody of anti-α tubulin indicated that ＂U＂ or ＂V＂ shaped spindles connected the migratory and stationary pronuclei during the third prezygotic division.This observation accounts for the close localization between these two types of pronuclei.
文摘目的:检测MMP-2、BMP-2基因在口腔颌面部骨化性纤维瘤(Oral and Maxillofacial ossifying fibroma,OMOF)中的表达情况,分析其与肿瘤病理分型、临床分级之间的关系,并探讨其作为OMOF基因治疗靶基因的可能性。方法:采用免疫组织化学EnVision法检测27例OMOF标本及8例颌骨非肿瘤组织中MMP-2、BMP-2基因的表达,SPSS统计软件分析所得数据。结果:27例OMOF标本中9例BMP-2阳性表达(33.3%),18例MMP-2阳性表达(66.7%),均定位于细胞浆与细胞核;而8例非肿瘤骨组织无一例表达;MMP-2蛋白的表达与肿瘤大小、临床分型、患者年龄与性别均无关,BMP-2蛋白的表达与肿瘤大小及年龄、临床病理分型有关(P<0.05),但与患者性别无关。结论:MMP-2蛋白比BMP-2蛋白在OMOF组织中的表达更高,因此将其作为研究OMOF免疫治疗靶点具有一定可行性。
