Using the specific affinity of tubulin for colchicine and the strong absorption of tubulin to DEAE ion exchangers at neutral pH and moderate ionic strength,the amounts of tubulin in the brain from both mice and chicks...Using the specific affinity of tubulin for colchicine and the strong absorption of tubulin to DEAE ion exchangers at neutral pH and moderate ionic strength,the amounts of tubulin in the brain from both mice and chicks during different developing stages were measured by ~3H-colchicine assay (expressed as colchicine binding activity).The results show that the rate oftubulin synthesis reached a peak value during the critical period of brain development.This is exactly the period during which the organization and function of thyroid are being perfected.Besides,during breeding period,the difference of tubulin contents between male and female is significant(P<0.001).The synthesis of tubulin is strictly sex dependent(this phenomenon appeared only during sex maturation stage).It is suggested that sexual hormones might exert their effect on tubulin synthesis.展开更多
Thyroid hormone deficiency was created artificially in chick embryos by injecting propyl-thiouracil (PTU).The tubulin was quantified by ~3H-colchicine assay(expressed as colchicine binding activity).Thyroid hormone de...Thyroid hormone deficiency was created artificially in chick embryos by injecting propyl-thiouracil (PTU).The tubulin was quantified by ~3H-colchicine assay(expressed as colchicine binding activity).Thyroid hormone deficiency resulted in 16％ to 28％ decline of the tubulin level in the developing chick brain from 15 to 19 days of embryonic age as compared with the control,whereas the total protein amount was not significantly affected.It suggests that thyroid hormone specifically affected the synthesis of brain tubulin.展开更多
文摘Using the specific affinity of tubulin for colchicine and the strong absorption of tubulin to DEAE ion exchangers at neutral pH and moderate ionic strength,the amounts of tubulin in the brain from both mice and chicks during different developing stages were measured by ~3H-colchicine assay (expressed as colchicine binding activity).The results show that the rate oftubulin synthesis reached a peak value during the critical period of brain development.This is exactly the period during which the organization and function of thyroid are being perfected.Besides,during breeding period,the difference of tubulin contents between male and female is significant(P<0.001).The synthesis of tubulin is strictly sex dependent(this phenomenon appeared only during sex maturation stage).It is suggested that sexual hormones might exert their effect on tubulin synthesis.
文摘Thyroid hormone deficiency was created artificially in chick embryos by injecting propyl-thiouracil (PTU).The tubulin was quantified by ~3H-colchicine assay(expressed as colchicine binding activity).Thyroid hormone deficiency resulted in 16％ to 28％ decline of the tubulin level in the developing chick brain from 15 to 19 days of embryonic age as compared with the control,whereas the total protein amount was not significantly affected.It suggests that thyroid hormone specifically affected the synthesis of brain tubulin.
