A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study,cetyltrimethylammonium bromide（CTAB） was used to rever...A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study,cetyltrimethylammonium bromide（CTAB） was used to reverse electro-osmotic flow in order to shorten the analysis time. β-CD was added to optimize the separation further. The effects of separation potential,concentration of CTAB,buffer electrolyte and β-CD etc. were researched and the best analysis conditions were obtained. Ten kinds of amino acids were separated and detected directly in running medium of 15.0 mmol/L diethylamine/0.10 mmol/L H3BO3/0.10 mmol/L CTAB/0.25 mmol/L β-CD（pH=11.0） at the separation potential of -20 kV within 7 min. The correlation coefficients of amino acids are 0.991—0.999 in the linear range of 1.00×10 -6 —1.00×10 -3 mol/L,the limit of detection are 0.17—0.94 μmol/L（S/N=3）. [WT5HZ]展开更多
目的联合检测肺腺癌患者外周血中细胞角蛋白(cytokeratin,CK,肺腺癌循环肿瘤细胞阳性)和CD133基因(肺癌干细胞标志物)的表达,探讨二者在评估肺腺癌预后的临床价值。方法选取2014年6月至2016年6月昆明总医院心胸外科和宣威市第一人民医...目的联合检测肺腺癌患者外周血中细胞角蛋白(cytokeratin,CK,肺腺癌循环肿瘤细胞阳性)和CD133基因(肺癌干细胞标志物)的表达,探讨二者在评估肺腺癌预后的临床价值。方法选取2014年6月至2016年6月昆明总医院心胸外科和宣威市第一人民医院心胸外科的178例未化疗的肺腺癌手术患者,中位年龄59岁,其中男性78例,女性100例。收集患者术前外周血标本10 m L,细胞块法和CK(免疫细胞化学法)用于检测CTC,PCR法用于检测CD133表达。免疫组化法检测肺腺癌组织中CK、Vimentin(Vim)、TTF-1、Ki-67、CD133的表达,并分析与外周血CK和CD133表达的关系。根据外周血CK和CD133的检测结果,将病例分为CK/CD133双阳性组和非双阳性组,统计学分析两组间临床病理因素的差异。结果 178例肺腺癌患者外周血CTC(CK阳性)的检出率为48.3%(86/178),与癌组织中Ki-67、Vim表达显著正相关(P<0.05);外周血的CD133阳性表达率为66.9%(119/178),与癌组织Vim、CD133表达显著正相关(P<0.05);外周血CK/CD133双阳组53例(29.8%),双阳组淋巴结转移率显著增高,肺腺癌组织学分化级别显著降低,临床分期显著提高,转移率显著增高(P<0.05)。结论肺腺癌患者外周血中CK与CD133双阳性表达提示外周血存在干性特征的循环肿瘤细胞,表明预后不良,联合检测CK与CD133可用于肺腺癌患者预后监测和指导治疗。展开更多
文摘A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study,cetyltrimethylammonium bromide（CTAB） was used to reverse electro-osmotic flow in order to shorten the analysis time. β-CD was added to optimize the separation further. The effects of separation potential,concentration of CTAB,buffer electrolyte and β-CD etc. were researched and the best analysis conditions were obtained. Ten kinds of amino acids were separated and detected directly in running medium of 15.0 mmol/L diethylamine/0.10 mmol/L H3BO3/0.10 mmol/L CTAB/0.25 mmol/L β-CD（pH=11.0） at the separation potential of -20 kV within 7 min. The correlation coefficients of amino acids are 0.991—0.999 in the linear range of 1.00×10 -6 —1.00×10 -3 mol/L,the limit of detection are 0.17—0.94 μmol/L（S/N=3）. [WT5HZ]
文摘目的联合检测肺腺癌患者外周血中细胞角蛋白(cytokeratin,CK,肺腺癌循环肿瘤细胞阳性)和CD133基因(肺癌干细胞标志物)的表达,探讨二者在评估肺腺癌预后的临床价值。方法选取2014年6月至2016年6月昆明总医院心胸外科和宣威市第一人民医院心胸外科的178例未化疗的肺腺癌手术患者,中位年龄59岁,其中男性78例,女性100例。收集患者术前外周血标本10 m L,细胞块法和CK(免疫细胞化学法)用于检测CTC,PCR法用于检测CD133表达。免疫组化法检测肺腺癌组织中CK、Vimentin(Vim)、TTF-1、Ki-67、CD133的表达,并分析与外周血CK和CD133表达的关系。根据外周血CK和CD133的检测结果,将病例分为CK/CD133双阳性组和非双阳性组,统计学分析两组间临床病理因素的差异。结果 178例肺腺癌患者外周血CTC(CK阳性)的检出率为48.3%(86/178),与癌组织中Ki-67、Vim表达显著正相关(P<0.05);外周血的CD133阳性表达率为66.9%(119/178),与癌组织Vim、CD133表达显著正相关(P<0.05);外周血CK/CD133双阳组53例(29.8%),双阳组淋巴结转移率显著增高,肺腺癌组织学分化级别显著降低,临床分期显著提高,转移率显著增高(P<0.05)。结论肺腺癌患者外周血中CK与CD133双阳性表达提示外周血存在干性特征的循环肿瘤细胞,表明预后不良,联合检测CK与CD133可用于肺腺癌患者预后监测和指导治疗。
