Summary： Glycoprotein （GP） Ibα ectodomain shedding has important implications for thrombosis and hemostasis. A disintegrin and metalloproteinase 17 （ADAM17） was identified to play an essen- tial role in agonist ...Summary： Glycoprotein （GP） Ibα ectodomain shedding has important implications for thrombosis and hemostasis. A disintegrin and metalloproteinase 17 （ADAM17） was identified to play an essen- tial role in agonist induced GPIbαshedding. The relationship of GPIbu shedding and ADAM 17 in the acute stage of atherosclerotic ischemic stroke （AIS） patients has not been thoroughly studied. A total of 306 patients and 230 controls matched for age, sex, race, history of hypertension and diabetes mel- litus were enrolled in the study. GPIbα, ADAM17, glycocalicin were detected by flow cytometry, Western blotting, and enzyme-linked immunosorbent assay （ELISA） respectively. Compared with the control group, the expression of GPIbα in patients with acute ischemic stroke was significantly lower （P=0.000, P〈0.01）. Plasma glycocalicin and ADAM17 in AIS group were higher than those in con- trol group （P=0.699, P=0.000）. Pearson＇s analysis showed glycocalicin bore no correlation with GPIbu in AIS patients （r=0.095, P〉0.05）. GPIbα and National Institute of Health Stroke Scale （NIHSS） had negative correlation （r=-0.514, P〈0.01）. Our findings indicate that ADAM 17 may be a risk factor for ischemic stroke in Chinese and the expression of GPIbα can serve as a measure for stroke severity.展开更多
基金supported by the National Natural Science Foundation of China (No. 81072945)
文摘Summary： Glycoprotein （GP） Ibα ectodomain shedding has important implications for thrombosis and hemostasis. A disintegrin and metalloproteinase 17 （ADAM17） was identified to play an essen- tial role in agonist induced GPIbαshedding. The relationship of GPIbu shedding and ADAM 17 in the acute stage of atherosclerotic ischemic stroke （AIS） patients has not been thoroughly studied. A total of 306 patients and 230 controls matched for age, sex, race, history of hypertension and diabetes mel- litus were enrolled in the study. GPIbα, ADAM17, glycocalicin were detected by flow cytometry, Western blotting, and enzyme-linked immunosorbent assay （ELISA） respectively. Compared with the control group, the expression of GPIbα in patients with acute ischemic stroke was significantly lower （P=0.000, P〈0.01）. Plasma glycocalicin and ADAM17 in AIS group were higher than those in con- trol group （P=0.699, P=0.000）. Pearson＇s analysis showed glycocalicin bore no correlation with GPIbu in AIS patients （r=0.095, P〉0.05）. GPIbα and National Institute of Health Stroke Scale （NIHSS） had negative correlation （r=-0.514, P〈0.01）. Our findings indicate that ADAM 17 may be a risk factor for ischemic stroke in Chinese and the expression of GPIbα can serve as a measure for stroke severity.
