本文报告人血浆LEK的RIA测定法,用TG—ECDI—LEK背部皮内多点免疫家兔,得高灵敏度、高特异性的ALS,工作稀度可达1:20,000,最小检出量5pg,多种肽类均无明显干扰,适用于血浆中检出微量LEK。作者用有效的样品抽提方法:快速冷分离出血浆,迅...本文报告人血浆LEK的RIA测定法,用TG—ECDI—LEK背部皮内多点免疫家兔,得高灵敏度、高特异性的ALS,工作稀度可达1:20,000,最小检出量5pg,多种肽类均无明显干扰,适用于血浆中检出微量LEK。作者用有效的样品抽提方法:快速冷分离出血浆,迅速的酸化及杆菌肽、抑肽酶等有效地防止了EK的降解,酸化血浆LEK被XAD—2树脂吸附和用水合甲醇洗脱。本法测定人血浆LEK平均为127.9±54pg/ml(m±S. E. M., n=61),范围45—200pg/ml。多种考验证明本法可靠。此法为循环中研究EK动力变化提供了条件。展开更多
Intracellular free calcium of platelets and serum free calcium were studied in human essential hypertension. Intracellular free calcium of platelets was significantly higher in hypertensive patients than that in normo...Intracellular free calcium of platelets and serum free calcium were studied in human essential hypertension. Intracellular free calcium of platelets was significantly higher in hypertensive patients than that in normotensive subjects, and this was correlated with blood pressure. There was no difference of serum free calcium between hypertensives and normotensive controls. Antihypertensive treatment with nifedipine resulted in a reduction of platelet free calcium, and this was correlated with the fall of blood pressure. In patients treated with clonidine, although there was no difference of platelet free calcium between hypertensives and normotensive controls, serum free clacium was significantly reduced. These results indicated that intracellular free calcium may be regulated by same hormonal or pharmacological factors which determined the height of blood pressure, calcium channel blockers may be more effective in prevention of cardiovascular and cerebrovascular complications caused by platelet hyperfunction in essential hypertension.展开更多
文摘本文报告人血浆LEK的RIA测定法,用TG—ECDI—LEK背部皮内多点免疫家兔,得高灵敏度、高特异性的ALS,工作稀度可达1:20,000,最小检出量5pg,多种肽类均无明显干扰,适用于血浆中检出微量LEK。作者用有效的样品抽提方法:快速冷分离出血浆,迅速的酸化及杆菌肽、抑肽酶等有效地防止了EK的降解,酸化血浆LEK被XAD—2树脂吸附和用水合甲醇洗脱。本法测定人血浆LEK平均为127.9±54pg/ml(m±S. E. M., n=61),范围45—200pg/ml。多种考验证明本法可靠。此法为循环中研究EK动力变化提供了条件。
文摘Intracellular free calcium of platelets and serum free calcium were studied in human essential hypertension. Intracellular free calcium of platelets was significantly higher in hypertensive patients than that in normotensive subjects, and this was correlated with blood pressure. There was no difference of serum free calcium between hypertensives and normotensive controls. Antihypertensive treatment with nifedipine resulted in a reduction of platelet free calcium, and this was correlated with the fall of blood pressure. In patients treated with clonidine, although there was no difference of platelet free calcium between hypertensives and normotensive controls, serum free clacium was significantly reduced. These results indicated that intracellular free calcium may be regulated by same hormonal or pharmacological factors which determined the height of blood pressure, calcium channel blockers may be more effective in prevention of cardiovascular and cerebrovascular complications caused by platelet hyperfunction in essential hypertension.
